Time Course Analysis of Expansion of TNC and CFU-C in Suspension Culture
| Cytokines . | Day 7 . | Day 14 . | Day 21 . | |||
|---|---|---|---|---|---|---|
| . | TNC . | CFU-C . | TNC . | CFU-C . | TNC . | CFU-C . |
| FL, KL | 3.8 × 103 | 970 | 1.6 × 104 | 180 | 1.5 × 104 | 30 |
| FL, IL-11 | 5.6 × 104 | 8,600 | 4.5 × 106 | 53,000 | 4.0 × 107 | 232,200 |
| KL, IL-11 | 8.6 × 105 | 105,300 | 2.2 × 108 | 453,800 | 5.2 × 108 | 36,300 |
| Cytokines . | Day 7 . | Day 14 . | Day 21 . | |||
|---|---|---|---|---|---|---|
| . | TNC . | CFU-C . | TNC . | CFU-C . | TNC . | CFU-C . |
| FL, KL | 3.8 × 103 | 970 | 1.6 × 104 | 180 | 1.5 × 104 | 30 |
| FL, IL-11 | 5.6 × 104 | 8,600 | 4.5 × 106 | 53,000 | 4.0 × 107 | 232,200 |
| KL, IL-11 | 8.6 × 105 | 105,300 | 2.2 × 108 | 453,800 | 5.2 × 108 | 36,300 |
One thousand Lin−Sca-1+c-kit+ cells were cultured in the presence of the designated cytokines. On day 7 and day 14 of culture, the cells were washed and then diluted in freshly prepared media, and the incubation continued for 7 more days. At each time of replating, aliquots were analyzed for colony formation.