Effect of O−2⋅ and NO Inhibitors on the Susceptibility of U937 and RU Clones to H-1 Virus Infection
| Pretreatment . | Cell Survival to H-1 Virus Infection (%) . | Recovered Virus (pfu/cell) . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | U937 . | RU1 . | RU2 . | RU3 . | RU4 . | U937 . | RU1 . | RU2 . | RU3 . | RU4 . |
| None | 0.14 | 93 | 90 | 98 | 91 | 90 | 0.20 | 0.72 | 0.23 | 0.11 |
| Allopurinol (3 mmol/L) | 0.16 | 62 | 57 | 49 | 31 | 77 | 3.1 | 5.4 | 2.4 | 2.8 |
| L-NMA (0.5 mmol/L) | 0.21 | 77 | 64 | 62 | 57 | 95 | 2.3 | 4.7 | 3.2 | 2.1 |
| Allopurinol + L-NMA | 0.13 | 53 | 45 | 44 | 22 | 79 | 3.9 | 6.0 | 3.3 | 3.2 |
| Pretreatment . | Cell Survival to H-1 Virus Infection (%) . | Recovered Virus (pfu/cell) . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | U937 . | RU1 . | RU2 . | RU3 . | RU4 . | U937 . | RU1 . | RU2 . | RU3 . | RU4 . |
| None | 0.14 | 93 | 90 | 98 | 91 | 90 | 0.20 | 0.72 | 0.23 | 0.11 |
| Allopurinol (3 mmol/L) | 0.16 | 62 | 57 | 49 | 31 | 77 | 3.1 | 5.4 | 2.4 | 2.8 |
| L-NMA (0.5 mmol/L) | 0.21 | 77 | 64 | 62 | 57 | 95 | 2.3 | 4.7 | 3.2 | 2.1 |
| Allopurinol + L-NMA | 0.13 | 53 | 45 | 44 | 22 | 79 | 3.9 | 6.0 | 3.3 | 3.2 |
Cultures (2 × 105 cells) pretreated for 24 hours with indicated agents were infected with H-1 virus at an MOI of 5 pfu/cell and further grown in the presence of the respective drug. Cultures were processed for the measurement of cell survival and virus production at 2 days postinfection. Survival of H-1 virus-infected cells is expressed relative to similarly pretreated but mock-infected cultures. Average values from 3 experiments are given (SD < 25%).