Abrogation of Spontaneous CFU-GM Growth by Depletion of Monocytes or T Lymphocytes
| . | Spontaneous Growth . | +GM-CSF . |
|---|---|---|
| MNCs | 13.3 ± 1.1 | 20.7 ± 1.7 |
| CD14− | 0.0 ± 0.0 | 23.3 ± 2.1 |
| CD3− | 0.9 ± 0.3 | 48.2 ± 2.4 |
| . | Spontaneous Growth . | +GM-CSF . |
|---|---|---|
| MNCs | 13.3 ± 1.1 | 20.7 ± 1.7 |
| CD14− | 0.0 ± 0.0 | 23.3 ± 2.1 |
| CD3− | 0.9 ± 0.3 | 48.2 ± 2.4 |
Unseparated PB-MNCs (2 × 105), CD14-depleted PB-MNCs (2 × 105), and CD3-depleted PB-MNCs (2 × 105) were cultured in triplicate in methylcellulose containing 30% FCS and IMDM. In each cell preparation, the presence of progenitor cells capable of colony formation was shown by the addition of GM-CSF (100 U/mL). Colony growth was assessed after 14 days. The mean colony numbers ± SEM of three independent experiments are given.