Specificity of Reduction in PKC Activity
| Treatment . | AChE . | PKC . | Non-PKC Kinase (pmol/min/mg) . |
|---|---|---|---|
| . | ( μmol/min/mg) . | (pmol/min/mg) . | . |
| Control | 3.76 ± 0.16 | 21.21 ± 0.36 | 1.86 ± 0.30 |
| RBC + PMA | 3.80 ± 0.38 | 71.90 ± 8.63 | 4.85 ± 3.22 |
| Control IRBC | 4.56 ± 0.24 | 21.52 ± 6.85 | 23.7 ± 5.53 |
| IRBC + PMA | 4.11 ± 0.17 | 4.34 ± 2.33 | 31.6 ± 1.86 |
| Treatment . | AChE . | PKC . | Non-PKC Kinase (pmol/min/mg) . |
|---|---|---|---|
| . | ( μmol/min/mg) . | (pmol/min/mg) . | . |
| Control | 3.76 ± 0.16 | 21.21 ± 0.36 | 1.86 ± 0.30 |
| RBC + PMA | 3.80 ± 0.38 | 71.90 ± 8.63 | 4.85 ± 3.22 |
| Control IRBC | 4.56 ± 0.24 | 21.52 ± 6.85 | 23.7 ± 5.53 |
| IRBC + PMA | 4.11 ± 0.17 | 4.34 ± 2.33 | 31.6 ± 1.86 |
AChE activity and kinase activity were measured in membrane fractions from infected (IRBC) (55%) and uninfected RBC with and without PMA treatment (500 nmol/L for 15 minutes at 37°C). AChE activity represents the mean of triplicate values ± SEM. The difference in activity between parasitized and uninfected cells is not significant. PKC activity represents activity in the presence of lipid activators, while non-PKC activity was measured in absence of lipid activators and the presence of excess pseudosubtrate peptide (as described in Materials and Methods). Values represent the mean of triplicate values ± SEM.