Data on Plasma Starting Material and Protein C Recovery During Purification of Normal and Patient Protein C by Anion Exchange Chromatography and BaSO4 Adsorption
| Material . | Patient . | Normal . | ||
|---|---|---|---|---|
| . | Portion 1 . | Portion 2 . | Portion 1 . | Portion 2 . |
| Plasma | ||||
| Volume (mL) | 230 | 260 | 200 | 400 |
| Protein C(immunological) concentration (arbitrary U/L) | 1.11 | 1.13 | 1.10 | 0.82 |
| Recovery of protein C(immunological) (%) | ||||
| First anion exchange chromatography step, eluate | 75 | 76 | 53 | 68 |
| BaSO4 eluate | 56 | 91 | 98 | 65 |
| Second anion exchange chromatography step, eluate | 126 | 57 | 76 | 85 |
| Material . | Patient . | Normal . | ||
|---|---|---|---|---|
| . | Portion 1 . | Portion 2 . | Portion 1 . | Portion 2 . |
| Plasma | ||||
| Volume (mL) | 230 | 260 | 200 | 400 |
| Protein C(immunological) concentration (arbitrary U/L) | 1.11 | 1.13 | 1.10 | 0.82 |
| Recovery of protein C(immunological) (%) | ||||
| First anion exchange chromatography step, eluate | 75 | 76 | 53 | 68 |
| BaSO4 eluate | 56 | 91 | 98 | 65 |
| Second anion exchange chromatography step, eluate | 126 | 57 | 76 | 85 |
Protein C was purified as described in Materials and Methods by anion exchange chromatography at pH 7.5 using 0.4 mol/L NaCl for elution followed by adsorption to and elution from BaSO4 by 0.1 mol/L Na citrate, pH 5.8, and a second anion exchange chromatography at pH 6.5 using a linear gradient from 0.18 to 0.50 mol/L NaCl. Each recovery value refers to the total amount of protein C(immunological) obtained from the previous purification step. One arbitrary unit of protein C(immunological) corresponds to the average amount of protein C in 1 L normal plasma.