Linearity of FVIIa ELISA Examined by Dilution of Patient Sample With High Levels of Recombinant FVIIa
| Diluent . | Dilution Factor 1/.. . | Determined FVIIa (ng/mL) . | Calculated FVIIa (ng/mL) . |
|---|---|---|---|
| PBS/casein | 3 | 2.64 | 7.93 |
| 9 | 0.89 | 8.01 | |
| 27 | 0.28 | 7.48 | |
| FVII-deficient plasma | 3 | 2.89 | 8.68 |
| 9 | 0.99 | 8.95 | |
| 27 | 0.33 | 8.88 |
| Diluent . | Dilution Factor 1/.. . | Determined FVIIa (ng/mL) . | Calculated FVIIa (ng/mL) . |
|---|---|---|---|
| PBS/casein | 3 | 2.64 | 7.93 |
| 9 | 0.89 | 8.01 | |
| 27 | 0.28 | 7.48 | |
| FVII-deficient plasma | 3 | 2.89 | 8.68 |
| 9 | 0.99 | 8.95 | |
| 27 | 0.33 | 8.88 |
A plasma sample from a patient infused with recombinant FVIIa was diluted in PBS/casein buffer or FVII-deficient plasma. FVIIa levels were determined in the sample dilutions using the ELISA and were then corrected for the dilution.