Population of the BM and PB of BM-SCID-hu Mice and Liv-SCID-hu Mice With Human Cells Transduced With Retroviral Vectors
| Mouse No. . | Tissue . | Vector . | Time After Transplant (mo) . | BM . | PB . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | %CD45+ . | %CD34+ . | CFU-GM . | BFU-E . | % Colonies neo Positive . | %CD45+ . | neo Positive . |
| 184c2 | HFBM | MY2 | 3 | 61 | 13 | 92 | 3 | 20 | 2 | neg |
| 184c4 | HFBM | MY2 | 3 | 47 | 16 | 172 | 18 | 50 | 23 | pos |
| 182d3 | HFBM | MY2 | 3 | 85 | 14 | 155 | 20 | 30 | 20 | pos |
| 182d5 | HFL | MY2 | 3 | 17 | 1 | 51 | 0 | 30 | 8 | neg |
| 181c4 | HFBM | LN | 4 | 25 | 3 | 272 | 35 | 40 | 9 | neg |
| 179b1 | HFL | LN | 4 | 58 | 10 | 140 | 7 | 30 | 30 | neg |
| 203-5 | HFL | MY2 | 6 | 14 | 1 | 17 | 0 | 10 | 5 | pos |
| 204-2 | HFL | MY2 | 6 | 20 | 5 | 270 | 0 | 10 | 1 | neg |
| 204-3 | HFL | MY2 | 6 | 68 | 13 | 179 | 15 | 30 | 31 | pos |
| 197a2 | HFBM | MY2 | 7 | 74 | 18 | 135 | 4 | 0 | 38 | pos |
| 197a5 | HFBM | MY2 | 7 | 30 | 7 | 110 | 0 | 40 | 6 | neg |
| Mouse No. . | Tissue . | Vector . | Time After Transplant (mo) . | BM . | PB . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | %CD45+ . | %CD34+ . | CFU-GM . | BFU-E . | % Colonies neo Positive . | %CD45+ . | neo Positive . |
| 184c2 | HFBM | MY2 | 3 | 61 | 13 | 92 | 3 | 20 | 2 | neg |
| 184c4 | HFBM | MY2 | 3 | 47 | 16 | 172 | 18 | 50 | 23 | pos |
| 182d3 | HFBM | MY2 | 3 | 85 | 14 | 155 | 20 | 30 | 20 | pos |
| 182d5 | HFL | MY2 | 3 | 17 | 1 | 51 | 0 | 30 | 8 | neg |
| 181c4 | HFBM | LN | 4 | 25 | 3 | 272 | 35 | 40 | 9 | neg |
| 179b1 | HFL | LN | 4 | 58 | 10 | 140 | 7 | 30 | 30 | neg |
| 203-5 | HFL | MY2 | 6 | 14 | 1 | 17 | 0 | 10 | 5 | pos |
| 204-2 | HFL | MY2 | 6 | 20 | 5 | 270 | 0 | 10 | 1 | neg |
| 204-3 | HFL | MY2 | 6 | 68 | 13 | 179 | 15 | 30 | 31 | pos |
| 197a2 | HFBM | MY2 | 7 | 74 | 18 | 135 | 4 | 0 | 38 | pos |
| 197a5 | HFBM | MY2 | 7 | 30 | 7 | 110 | 0 | 40 | 6 | neg |
HFBM or HFL was transduced with a neo containing vector (either LN or MY2) and then transplanted into SCID mice. At the indicated time, the mouse BM and PB were analyzed. The percentage of human CD45+ and CD34+ cells was determined by flow cytometry and the number of human CFU-GM and BFU-E (presented as number of colonies/5 × 104 human cells plated) was measured by clonogenic assays. Incorporated neo gene was detected in colonies and the peripheral blood by PCR analysis with neo-specific primers followed by a Southern blot with an intervening sequence-specific probe. The data for the BM are presented as % of neo-positive colonies based on the analysis of 10 randomly picked colonies and the data for the PB are presented as detection of neo sequences in lysed PBMC (pos) or as no detection of neo sequences in lysed PBMC (neg).