Long-Term Population of Mouse Bone Marrow With Human Precursor Cells
| Mouse No. . | Time After Transplant (Mo.) . | % Cells Positive for Human CD . | No. Human Colonies . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | 45+ . | 34+/10− . | 34+/10+ . | 19+/20− . | 19+/20+ . | 13+/33+ . | CFU-GM . | BFU-e . | CFU-mix . |
| 189c6 | 7 | 89 | 2 | 19 | 45 | 43 | 50 | 89 | 11 | 3 |
| 192c3 | 7 | 25 | 2 | 3 | 10 | 10 | 7 | 242 | 3 | 0 |
| 193b1 | 7 | 66 | 2 | 9 | 25 | 19 | 63 | 112 | 50 | 5 |
| 187c5 | 8 | 86 | 1 | 15 | 51 | 29 | 44 | 72 | 23 | 2 |
| 187c6 | 8 | 18 | 1 | 1 | 7 | 6 | 6 | 136 | 7 | 0 |
| 187c7 | 8 | 23 | 1 | 3 | 12 | 8 | 1 | 134 | 5 | 0 |
| 188a2 | 8 | 77 | 4 | 17 | 45 | 29 | 43 | 122 | 27 | 3 |
| Mouse No. . | Time After Transplant (Mo.) . | % Cells Positive for Human CD . | No. Human Colonies . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | 45+ . | 34+/10− . | 34+/10+ . | 19+/20− . | 19+/20+ . | 13+/33+ . | CFU-GM . | BFU-e . | CFU-mix . |
| 189c6 | 7 | 89 | 2 | 19 | 45 | 43 | 50 | 89 | 11 | 3 |
| 192c3 | 7 | 25 | 2 | 3 | 10 | 10 | 7 | 242 | 3 | 0 |
| 193b1 | 7 | 66 | 2 | 9 | 25 | 19 | 63 | 112 | 50 | 5 |
| 187c5 | 8 | 86 | 1 | 15 | 51 | 29 | 44 | 72 | 23 | 2 |
| 187c6 | 8 | 18 | 1 | 1 | 7 | 6 | 6 | 136 | 7 | 0 |
| 187c7 | 8 | 23 | 1 | 3 | 12 | 8 | 1 | 134 | 5 | 0 |
| 188a2 | 8 | 77 | 4 | 17 | 45 | 29 | 43 | 122 | 27 | 3 |
The numbers of myeloid precursors (CFU-GM) and erythroid precursors (BFU-E) present in the BM of BM-SCID-hu mice (192c3, 187c5, 187c6, 188a2, 189c6, and 193b1) and a Liv-SCID-hu mouse (187c7) were assessed using clonogenic assays as described in Materials and Methods. The mean percentage of human precursors/5 × 104 human cells is shown. Expression of the indicated human CD was determine by flow cytometry.