Analysis of Human Precursor Cells Present in the BM of BM-SCID-hu Mice and Liv-SCID-hu Mice
| . | CFU-GM . | BFU-E . | CFU-Mix . |
|---|---|---|---|
| BM-SCID-hu mouse | |||
| 144a5 | 47 | 4 | 0 |
| 144c1 | 43 | 11 | 0 |
| 149c1 | 62 | 14 | 2 |
| 149c2 | 45 | 16 | 0 |
| Mean (± SEM) | 49.2 ± 4.3 | 11.3 ± 2.6 | 0.5 ± 0.5 |
| Liv-SCID-hu mouse | |||
| 144c2 | 3 | 2 | 0 |
| 144c3 | 31 | 2 | 0 |
| 144c6 | 104 | 60 | 0 |
| 141a3 | 94 | 19 | 0 |
| 141a5 | 45 | 8 | 2 |
| 149c3 | 79 | 49 | 0 |
| 149c4 | 82 | 38 | 1 |
| Mean (± SEM) | 62.6 ± 13.9 | 25.4 ± 8.9 | 0.4 ± 0.3 |
| Control HFBM No. | |||
| 1 | 54 | 87 | 2 |
| 2 | 61 | 22 | 2 |
| 3 | 48 | 108 | 5 |
| 4 | 61 | 87 | 12 |
| Mean (± SEM) | 56.0 ± 3.1 | 76.0 ± 18.7 | 5.3 ± 2.4 |
| Control HFL No. | |||
| 1 | 67 | 62 | 10 |
| 2 | 96 | 60 | 6 |
| 3 | 65 | 22 | 14 |
| 4 | 53 | 8 | 6 |
| 5 | 96 | 60 | 6 |
| Mean (± SEM) | 75.4 ± 19.6 | 42.4 ± 25.5 | 8.3 ± 3.5 |
| . | CFU-GM . | BFU-E . | CFU-Mix . |
|---|---|---|---|
| BM-SCID-hu mouse | |||
| 144a5 | 47 | 4 | 0 |
| 144c1 | 43 | 11 | 0 |
| 149c1 | 62 | 14 | 2 |
| 149c2 | 45 | 16 | 0 |
| Mean (± SEM) | 49.2 ± 4.3 | 11.3 ± 2.6 | 0.5 ± 0.5 |
| Liv-SCID-hu mouse | |||
| 144c2 | 3 | 2 | 0 |
| 144c3 | 31 | 2 | 0 |
| 144c6 | 104 | 60 | 0 |
| 141a3 | 94 | 19 | 0 |
| 141a5 | 45 | 8 | 2 |
| 149c3 | 79 | 49 | 0 |
| 149c4 | 82 | 38 | 1 |
| Mean (± SEM) | 62.6 ± 13.9 | 25.4 ± 8.9 | 0.4 ± 0.3 |
| Control HFBM No. | |||
| 1 | 54 | 87 | 2 |
| 2 | 61 | 22 | 2 |
| 3 | 48 | 108 | 5 |
| 4 | 61 | 87 | 12 |
| Mean (± SEM) | 56.0 ± 3.1 | 76.0 ± 18.7 | 5.3 ± 2.4 |
| Control HFL No. | |||
| 1 | 67 | 62 | 10 |
| 2 | 96 | 60 | 6 |
| 3 | 65 | 22 | 14 |
| 4 | 53 | 8 | 6 |
| 5 | 96 | 60 | 6 |
| Mean (± SEM) | 75.4 ± 19.6 | 42.4 ± 25.5 | 8.3 ± 3.5 |
The numbers of myeloid precursors (CFU-GM) and erythroid precursors (BFU-E) present in the BM of BM-SCID-hu mice (n = 4 mice) and Liv-SCID-hu mice (n = 7 mice) and fetal BM (n = 4 fetuses) and fetal liver (n = 5 fetuses) were assessed as described in Materials and Methods. The mean number of human precursor cells/5 × 104 human cells in duplicate clonogenic assays is shown.