Effect of T Cells Stimulated With IL-2 or DC and IL-2 on Colony Growth by Autologous CML or MHC-Matched Normal BMMNCs
| Effector Cell Type . | Autologous CML Targets . | HLA-Matched Normal BM Targets . |
|---|---|---|
| None | ||
| Patient no. 1 | 107 ± 22 | 44 ± 9 |
| Patient no. 2 | 213 ± 31 | 79 ± 20 |
| Patient no. 3 | 332 ± 145 | 169 ± 73 |
| IL-2 expanded | ||
| Patient no. 1 | 94 ± 44 | 36 ± 20 |
| Patient no. 2 | 116 ± 34 | 51 ± 12 |
| Patient no. 3 | 245 ± 62 | 117 ± 16 |
| P ≤ .01 | P < NS | |
| DC + IL-2 expanded | ||
| Patient no. 1 | 16 ± 10 | 34 ± 16 |
| Patient no. 2 | 23 ± 10 | 58 ± 3 |
| Patient no. 3 | 31 ± 1 | 212 ± 110 |
| P ≤ .001 | P = NS |
| Effector Cell Type . | Autologous CML Targets . | HLA-Matched Normal BM Targets . |
|---|---|---|
| None | ||
| Patient no. 1 | 107 ± 22 | 44 ± 9 |
| Patient no. 2 | 213 ± 31 | 79 ± 20 |
| Patient no. 3 | 332 ± 145 | 169 ± 73 |
| IL-2 expanded | ||
| Patient no. 1 | 94 ± 44 | 36 ± 20 |
| Patient no. 2 | 116 ± 34 | 51 ± 12 |
| Patient no. 3 | 245 ± 62 | 117 ± 16 |
| P ≤ .01 | P < NS | |
| DC + IL-2 expanded | ||
| Patient no. 1 | 16 ± 10 | 34 ± 16 |
| Patient no. 2 | 23 ± 10 | 58 ± 3 |
| Patient no. 3 | 31 ± 1 | 212 ± 110 |
| P ≤ .001 | P = NS |
One hundred thousand BMMNCs, were incubated with 25 × 105 T cells for 6 hours before culture for 10 days in methylcellulose medium. Values represent the mean number of colonies ± SD of triplicate cultures. Statistical significance was determined using the Student's t-test.
Abbreviation: NS, not significant (P ≥ .05).