Table 1. Effect of Sorted CD34 Bone... | American Society of Hematology

Table 1.

Effect of Sorted CD34⁺ Bone Marrow Cells on IL-6 Production by Human Osteoblast Cells

Osteoblast Isolate	No CD34⁺ Cells	1 × 10⁴ CD34⁺ Cells
1	10,131 ± 635	16,230 ± 832*
2	12,072 ± 1,819	18,987 ± 2,453^†
3	4,204 ± 338	17,006 ± 4,253^†

Osteoblast Isolate	No CD34⁺ Cells	1 × 10⁴ CD34⁺ Cells
1	10,131 ± 635	16,230 ± 832*
2	12,072 ± 1,819	18,987 ± 2,453^†
3	4,204 ± 338	17,006 ± 4,253^†

Nonadherent bone marrow mononuclear CD34⁺ cells were isolated by positive immunomagnetic selection and prepared for fluorescence-activated cell sorting using a phycoerythrin conjugate of the anti-CD34 antibody, HPCA-2 (Becton Dickinson, San Jose, CA). Selected CD34⁺ cells (1.0 × 10⁴) were seeded directly onto confluent human osteoblast monolayers in 96-well tissue culture plates containing 1% FBS. At 96 hours, conditioned medium was collected and assayed for IL-6 by ELISA. Data from a representative of two independent experiments are presented as the mean ± standard deviation for triplicate determinations.

Significant difference from no CD34⁺ cells (osteoblasts only) to a statistical significance of P < .05.

†

Significant difference from no CD34⁺ cells (osteoblasts only) to a statistical significance of P < .01.

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