Table 2. Neutralization and... | American Society of Hematology

Table 2.

Neutralization and Immunoprecipitation Assays of Plasmas From 23 Hemophilic Inhibitor Patients Treated With Plasma-Derived fVIII

Inhibitor	Percent Neutralization by FVIII Region				Major Inhibitor	Immunoprecipitation
	A2	C2	peptide	LCh	Epitope(s) Domain(s)	of
			355-362			A2	C2
CHA	≥95	—	—	≤5	A2	+	−
RC	≥95	—	—	≤5	A2	+	−
RM	86	—	≤5	≤10	A2	+	−
L	≤10	≥95	—	≥66	C2	+	+
WD	56	≤10	59	34	A2, pep 341-63	+	+
RJ	≥95	14	—	21	A2, C2	+	+
RDU	70	28	—	27	A2, C2	+	+
CC	67	37	—	39	A2, C2	+	+
RMA	62	39	—	41	A2, C2	+	+
KB	≤5	33	—	≥95	C2, AR3-A3-C1	+	+
YA	≤10	62	—	≥95	C2, AR3-A3-C1	+	+
MP	≤5	20	—	≥95	C2, AR3-A3-C1	+	+
GK1832	≤5	31	—	≥95	C2, AR3-A3-C1	+	+
MU	≤5	15	—	94	C2, AR3-A3-C1	+	+
SCN	≤5	33	—	88	C2, AR3-A3-C1	+	+
GK1824	≤5	40	≤5	≥95	C2, AR3-A3-C1	+	+
RI	≤10	26	—	70	C2, AR3-A3-C1	+	+
JR	31	29	—	68	A2, C2, AR3-A3-C1	+	+
WG	42	26	—	67	A2, C2, AR3-A3-C1	+	+
HG	50	25	—	40	A2, C2, AR3-A3-C1	+	+
GK1833	12	28	—	87	A2, C2, AR3-A3-C1	+	+
MS	27	30	—	78	A2, C2, AR3-A3-C1	+	+
GK1831	40	19	—	56	A2, C2, AR3-A3-C1	+	+

Inhibitor	Percent Neutralization by FVIII Region				Major Inhibitor	Immunoprecipitation
	A2	C2	peptide	LCh	Epitope(s) Domain(s)	of
			355-362			A2	C2
CHA	≥95	—	—	≤5	A2	+	−
RC	≥95	—	—	≤5	A2	+	−
RM	86	—	≤5	≤10	A2	+	−
L	≤10	≥95	—	≥66	C2	+	+
WD	56	≤10	59	34	A2, pep 341-63	+	+
RJ	≥95	14	—	21	A2, C2	+	+
RDU	70	28	—	27	A2, C2	+	+
CC	67	37	—	39	A2, C2	+	+
RMA	62	39	—	41	A2, C2	+	+
KB	≤5	33	—	≥95	C2, AR3-A3-C1	+	+
YA	≤10	62	—	≥95	C2, AR3-A3-C1	+	+
MP	≤5	20	—	≥95	C2, AR3-A3-C1	+	+
GK1832	≤5	31	—	≥95	C2, AR3-A3-C1	+	+
MU	≤5	15	—	94	C2, AR3-A3-C1	+	+
SCN	≤5	33	—	88	C2, AR3-A3-C1	+	+
GK1824	≤5	40	≤5	≥95	C2, AR3-A3-C1	+	+
RI	≤10	26	—	70	C2, AR3-A3-C1	+	+
JR	31	29	—	68	A2, C2, AR3-A3-C1	+	+
WG	42	26	—	67	A2, C2, AR3-A3-C1	+	+
HG	50	25	—	40	A2, C2, AR3-A3-C1	+	+
GK1833	12	28	—	87	A2, C2, AR3-A3-C1	+	+
MS	27	30	—	78	A2, C2, AR3-A3-C1	+	+
GK1831	40	19	—	56	A2, C2, AR3-A3-C1	+	+

LCh refers to domains AR3-A3–C1-C2 and AR3-A3–C1 to LCh epitope(s) outside C2. The greater neutralization of some plasmas by LCh than by C2 indicated that inhibitor epitopes exist both within C2 and AR3-A3-C1. The average maximal neutralization by the heavy chain plus the LCh polypeptides was 96% ± 8.8% standard deviation (SD).

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