List of the Primers
| Receptor Product . | Sequence (5′-3′) . | Reference . | PCR . | |
|---|---|---|---|---|
| Fc-γ RI | ||||
| Forward | CTG CAG GAG TGT CCA TCA CGG TGA AAG A | 350 bp | ||
| Reverse | GGA TGT GAA ACC AGA CAG GAG CTG ATG A | |||
| Fc-γ RII | ||||
| Forward (4) | GCT GGA GGA ACA AAC TAC TGA ACA G | 24 | β1 | 477 bp |
| Reverse (2) | GCA GCT TCT TCC AGA TCA GGA GGA | β2 | 339 bp | |
| Fc-γ RIII | ||||
| Forward (1) | AGT CAC AGT GGG GAC TAC TAC TGC A | 24 | 268 bp | |
| Reverse (3) | CAC TTG TCT TGA GGA GCC TGG TGC T | |||
| γ-Chain | ||||
| Forward | CTC AGC CGT GAT CTT GTT CTT GC | 24 | 238 bp | |
| Reverse | GCT TCA GAG TCT CAT ATG TCT CC | |||
| Fc-ɛ RII/CD23 | ||||
| Forward | GCA CGC CTC ATC ACT GAA AGG | 44 | 1,033 bp | |
| Reverse | GGG TTC ACT TTT TGG GGT | |||
| Mac-2 | ||||
| Forward | GCC CCG CAT GCT GAT CAC AAT C | 288 bp | ||
| Reverse | GTT GTA CTG CAG TAG GTG AGC ATC GT | |||
| Fc-ɛ RIα | ||||
| Forward | GAT CCA CAA TGG TAC CGT CTC TGA GG | 358 bp | ||
| Reverse | CCA CCT GCC TAA GAT AGC CCT TGC | |||
| CD2 | ||||
| Forward | GCG GAC TGC AGA GAC AAT GAG AC | 491 bp | ||
| Reverse | CGC CTC ACA CTT GAA TGG TGC | |||
| B29 | ||||
| Forward | TAA GTC TAG AAG TTC CGT GCC ACA GCT GTC | 24 | 320 bp | |
| Reverse | CAC TGA ATT CCC AAG GAA GCC CTT GTT CCC | |||
| MBP | ||||
| Forward | GAG CGT CTG CTC TTC ATC TGA | 387 bp | ||
| Reverse | CAC TGA AAC TGT GAA TGG AGG C | |||
| β-actin | ||||
| Forward | ATG GAT GAC GAT ATC GCT | 44 | 587 bp | |
| Reverse | ATG AGG TAG TCT GTC AGG T |
| Receptor Product . | Sequence (5′-3′) . | Reference . | PCR . | |
|---|---|---|---|---|
| Fc-γ RI | ||||
| Forward | CTG CAG GAG TGT CCA TCA CGG TGA AAG A | 350 bp | ||
| Reverse | GGA TGT GAA ACC AGA CAG GAG CTG ATG A | |||
| Fc-γ RII | ||||
| Forward (4) | GCT GGA GGA ACA AAC TAC TGA ACA G | 24 | β1 | 477 bp |
| Reverse (2) | GCA GCT TCT TCC AGA TCA GGA GGA | β2 | 339 bp | |
| Fc-γ RIII | ||||
| Forward (1) | AGT CAC AGT GGG GAC TAC TAC TGC A | 24 | 268 bp | |
| Reverse (3) | CAC TTG TCT TGA GGA GCC TGG TGC T | |||
| γ-Chain | ||||
| Forward | CTC AGC CGT GAT CTT GTT CTT GC | 24 | 238 bp | |
| Reverse | GCT TCA GAG TCT CAT ATG TCT CC | |||
| Fc-ɛ RII/CD23 | ||||
| Forward | GCA CGC CTC ATC ACT GAA AGG | 44 | 1,033 bp | |
| Reverse | GGG TTC ACT TTT TGG GGT | |||
| Mac-2 | ||||
| Forward | GCC CCG CAT GCT GAT CAC AAT C | 288 bp | ||
| Reverse | GTT GTA CTG CAG TAG GTG AGC ATC GT | |||
| Fc-ɛ RIα | ||||
| Forward | GAT CCA CAA TGG TAC CGT CTC TGA GG | 358 bp | ||
| Reverse | CCA CCT GCC TAA GAT AGC CCT TGC | |||
| CD2 | ||||
| Forward | GCG GAC TGC AGA GAC AAT GAG AC | 491 bp | ||
| Reverse | CGC CTC ACA CTT GAA TGG TGC | |||
| B29 | ||||
| Forward | TAA GTC TAG AAG TTC CGT GCC ACA GCT GTC | 24 | 320 bp | |
| Reverse | CAC TGA ATT CCC AAG GAA GCC CTT GTT CCC | |||
| MBP | ||||
| Forward | GAG CGT CTG CTC TTC ATC TGA | 387 bp | ||
| Reverse | CAC TGA AAC TGT GAA TGG AGG C | |||
| β-actin | ||||
| Forward | ATG GAT GAC GAT ATC GCT | 44 | 587 bp | |
| Reverse | ATG AGG TAG TCT GTC AGG T |