Effect of TTP Plasma on PGI2 Production by Microvascular EC of Dermal and Pulmonary Lineage
| Incubation . | Normal Plasma . | TTP-2 Plasma . | TTP-3 Plasma . | |||
|---|---|---|---|---|---|---|
| . | Dermal . | Pulmonary . | Dermal . | Pulmonary . | Dermal . | Pulmonary . |
| Baseline (2 h) | 535 | 448 | 562 | 448 | 651 | 345 |
| 16 h | 2,417 (4.5) | 906 (2.0) | 1,797 (3.2) | 762 (1.7) | 1,697 (2.6) | 695 (2.0) |
| 16 h, stimulated | 1,743 (3.3) | 10,756 (24.0) | 1,904 (3.4) | 8,197 (18.3) | 1,718 (2.6) | 5,509 (16.0) |
| Incubation . | Normal Plasma . | TTP-2 Plasma . | TTP-3 Plasma . | |||
|---|---|---|---|---|---|---|
| . | Dermal . | Pulmonary . | Dermal . | Pulmonary . | Dermal . | Pulmonary . |
| Baseline (2 h) | 535 | 448 | 562 | 448 | 651 | 345 |
| 16 h | 2,417 (4.5) | 906 (2.0) | 1,797 (3.2) | 762 (1.7) | 1,697 (2.6) | 695 (2.0) |
| 16 h, stimulated | 1,743 (3.3) | 10,756 (24.0) | 1,904 (3.4) | 8,197 (18.3) | 1,718 (2.6) | 5,509 (16.0) |
Dermal (MVEC-2) or pulmonary (HMVEC-LU) microvascular EC were plated as 1 × 105 cells in 0.5 mL apoptosis medium and exposed to a 1:100 dilution of plasma from an HIV-seronegative normal donor or HIV-seronegative patients with active TTP (TTP-2 and -3). One-hundred–microliter aliquots of culture supernatant were taken at 2 and 16 hours of culture. The remaining culture medium was then discarded, 0.5 mL fresh apoptosis medium plus sodium arachidonate was added, and 100-μL samples were taken after 15 minutes of incubation at 37°C. PGI2 levels, in picograms per milliliter, were measured as described in the text. The fold increase is in parentheses.