Hematopoietic Chimerism of Recipient F1 Mice After Parental BM Grafting
Recipient . | Treatment3-150 . | Donor . | BM Cells3-151 . | Hematopoietic Chimerism3-152 . | |
---|---|---|---|---|---|
. | . | . | . | H-2b/b . | H-2b/d . |
B6D2F1 | None | B6 | 1 × 105 | 6.2% | 91.0% |
8.5% | 90.4% | ||||
16.5% | 82.4% | ||||
B6D2F1 | G-CSF | B6 | 1 × 105 | 42.0% | 55.0% |
54.0% | 40.3% | ||||
60.2% | 38.2% |
Recipient . | Treatment3-150 . | Donor . | BM Cells3-151 . | Hematopoietic Chimerism3-152 . | |
---|---|---|---|---|---|
. | . | . | . | H-2b/b . | H-2b/d . |
B6D2F1 | None | B6 | 1 × 105 | 6.2% | 91.0% |
8.5% | 90.4% | ||||
16.5% | 82.4% | ||||
B6D2F1 | G-CSF | B6 | 1 × 105 | 42.0% | 55.0% |
54.0% | 40.3% | ||||
60.2% | 38.2% |
G-CSF at 25 μg/kg/d was administered subcutaneously with a miniosmotic pump starting on day −14 and BM cells were grafted on day 0.
Number of T-cell–depleted B6 BM cells transplanted.
Hematopoietic chimerism was examined on day 40. Cells with H-2b/b and H-2b/d phenotypes correspond to donor and recipient, respectively.