Content and Kinetic Status of LTC-IC From G-CSF–Mobilized PB CD34+ Cells Compared With Steady-State BM Samples
| Source of Cells . | LTC-IC (×104 CD34+ cells) . | % S-Phase . | % Cycling . |
|---|---|---|---|
| PB | 48.2 ± 35 (1.2-152) | 1 ± 3 | 67 ± 10 |
| BM | 62.5 ± 54 (3.9-365) | 21 ± 8* | 75 ± 6 |
| Source of Cells . | LTC-IC (×104 CD34+ cells) . | % S-Phase . | % Cycling . |
|---|---|---|---|
| PB | 48.2 ± 35 (1.2-152) | 1 ± 3 | 67 ± 10 |
| BM | 62.5 ± 54 (3.9-365) | 21 ± 8* | 75 ± 6 |
The number of LTC-IC was evaluated after 5 weeks of liquid culture as described in the Materials and Methods. To determine the percentage of S-phase and cycling LTC-IC, the CD34+ cell suspension was incubated with ARA-C for 1 hour and 16 hours, respectively, before the establishment of liquid culture. The results are expressed as the mean ± SEM (range) of 16 and 10 different experiments from PB and BM samples, respectively.
Statistically significant compared with PB LTC-IC (P < .001).