Biochemical Characteristics of G6PD Variants
| Variant . | Class . | Enzyme Activity (%) . | Electro- . | Km G6P (μmol/L) . | Km NADP+ (μmol/L) . | Ki NADPH (μmol/L) . | 2-Deoxy G6P (%)* . | Gal-6P (%)* . | Deamino NADP+ (%)† . | pH Optimum . | Heat Stability . | Reference No. . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | phoresis (%) . | . | . | . | . | . | . | . | . | . |
| Musashino | 3 | 17.5 | 90 | 32 | 2.3 | 8.3 | 5.1 | 16.3 | 80 | Normal | Normal | 8 |
| Asahikawa | 1 | 3.8 | 98 | 30 | 18 | 2.1 | 7.1 | 11.9 | 109 | Biphasic | Labile | 9 |
| Iwate | 1 | 2.3 | 75 | 37 | 40 | 3.0 | 4.3 | 4.3 | 51 | Normal | Labile | 10 |
| Niigata | 1 | 1.8 | 38 | 47 | 7.3 | 2.3 | 32 | 540 | 37 | Acidic | Labile | 11 |
| Yamaguchi | 1 | 3.5 | 57 | 37 | 15 | 7.6 | 4.1 | 19.0 | 94 | Normal | Labile | 12 |
| Beverly Hills | 1 | <0.1 | 93 | 41 | 15 | ND | <4 | ND | 61 | Biphasic | Labile | 19 |
| Fukushima | 1 | 2.8 | 86 | 31 | 5.0 | 4.4 | 3.0 | 11.0 | 37 | Normal | Labile | 12 |
| Tokyo | 1 | 4.4 | 90 | 65 | 5.5 | ND | 2.5 | ND | 55 | Normal | Labile | 25 |
| Morioka | 1 | 4.5 | 100 | 30 | 3.4 | 19.4 | 6.1 | 5.3 | 81 | Normal | Labile | 13 |
| Santiago de Cuba | 1 | 5 | 80 | 50 | 43 | ND | <4 | ND | ND | ND | Labile | 29 |
| Kamiube | 3 | 24.5 | 100 | 57 | 3.0 | 8.0 | 3.7 | 11.0 | 52 | Normal | Normal | 14 |
| B (control) | 4 | 100 | 100 | 31-71 | 2.6-6.6 | 4.9-12.9 | 1.6-6.4 | 4.3-12.7 | 51-69 | Normal | Normal |
| Variant . | Class . | Enzyme Activity (%) . | Electro- . | Km G6P (μmol/L) . | Km NADP+ (μmol/L) . | Ki NADPH (μmol/L) . | 2-Deoxy G6P (%)* . | Gal-6P (%)* . | Deamino NADP+ (%)† . | pH Optimum . | Heat Stability . | Reference No. . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | phoresis (%) . | . | . | . | . | . | . | . | . | . |
| Musashino | 3 | 17.5 | 90 | 32 | 2.3 | 8.3 | 5.1 | 16.3 | 80 | Normal | Normal | 8 |
| Asahikawa | 1 | 3.8 | 98 | 30 | 18 | 2.1 | 7.1 | 11.9 | 109 | Biphasic | Labile | 9 |
| Iwate | 1 | 2.3 | 75 | 37 | 40 | 3.0 | 4.3 | 4.3 | 51 | Normal | Labile | 10 |
| Niigata | 1 | 1.8 | 38 | 47 | 7.3 | 2.3 | 32 | 540 | 37 | Acidic | Labile | 11 |
| Yamaguchi | 1 | 3.5 | 57 | 37 | 15 | 7.6 | 4.1 | 19.0 | 94 | Normal | Labile | 12 |
| Beverly Hills | 1 | <0.1 | 93 | 41 | 15 | ND | <4 | ND | 61 | Biphasic | Labile | 19 |
| Fukushima | 1 | 2.8 | 86 | 31 | 5.0 | 4.4 | 3.0 | 11.0 | 37 | Normal | Labile | 12 |
| Tokyo | 1 | 4.4 | 90 | 65 | 5.5 | ND | 2.5 | ND | 55 | Normal | Labile | 25 |
| Morioka | 1 | 4.5 | 100 | 30 | 3.4 | 19.4 | 6.1 | 5.3 | 81 | Normal | Labile | 13 |
| Santiago de Cuba | 1 | 5 | 80 | 50 | 43 | ND | <4 | ND | ND | ND | Labile | 29 |
| Kamiube | 3 | 24.5 | 100 | 57 | 3.0 | 8.0 | 3.7 | 11.0 | 52 | Normal | Normal | 14 |
| B (control) | 4 | 100 | 100 | 31-71 | 2.6-6.6 | 4.9-12.9 | 1.6-6.4 | 4.3-12.7 | 51-69 | Normal | Normal |