Table 2. Oligonucleotide V L PCR Primers... | American Society of Hematology

Table 2.

Oligonucleotide V_L PCR Primers

Primer	Location	Orientation	Sequence (5′-3′)
Vκ1&4	FR1	Sense	GACATCSWGATGACCCAGTCTCC
Vκ2&6	FR1	Sense	GAWRTTGTGMTGACTCAGTCTCC
Vκ3	FR1	Sense	GAAATTGTGTTGACGCAGTCTCC
Vκ5	FR1	Sense	GAAACGACACTCACGCAGTCTCC
Jκ1-4	FR4	Anti-sense	ACGTTTGATHTCCACYTTGGTCCC
Jκ5	FR4	Anti-sense	ACGTTTAATCTCCAGTCGTGTCCC
Vλ1	FR1	Sense	CAGTCTGTSBTGACKCAGCCRCCY
Vλ2	FR1	Sense	CAGTCTGCCCTGACTCAGCCTSSYT
Vλ3	FR1	Sense	TCYTMTGWGCTGACTCAGSMM
Vλ7&8	FR1	Sense	CAGRCTGTGGTGACYCAGGAGCCMTC
Vλ9	FR1	Sense	CAGCCTGTGCTGACTCAGCCACCTTC
JλC	FR4	Anti-sense	ACCKAGGACGGTSASCTKGGTSCC

Primer	Location	Orientation	Sequence (5′-3′)
Vκ1&4	FR1	Sense	GACATCSWGATGACCCAGTCTCC
Vκ2&6	FR1	Sense	GAWRTTGTGMTGACTCAGTCTCC
Vκ3	FR1	Sense	GAAATTGTGTTGACGCAGTCTCC
Vκ5	FR1	Sense	GAAACGACACTCACGCAGTCTCC
Jκ1-4	FR4	Anti-sense	ACGTTTGATHTCCACYTTGGTCCC
Jκ5	FR4	Anti-sense	ACGTTTAATCTCCAGTCGTGTCCC
Vλ1	FR1	Sense	CAGTCTGTSBTGACKCAGCCRCCY
Vλ2	FR1	Sense	CAGTCTGCCCTGACTCAGCCTSSYT
Vλ3	FR1	Sense	TCYTMTGWGCTGACTCAGSMM
Vλ7&8	FR1	Sense	CAGRCTGTGGTGACYCAGGAGCCMTC
Vλ9	FR1	Sense	CAGCCTGTGCTGACTCAGCCACCTTC
JλC	FR4	Anti-sense	ACCKAGGACGGTSASCTKGGTSCC