Number of Basophils Chemoattracted to the Inflamed Area After Injection With PBS, FMLP, LPS, RANTES, and MCP-1
| Treatment . | No. of Cells in 200 mm2 . | P< . |
|---|---|---|
| PBS (control) | 32 ± 12 | — |
| LPS 10 ng | 193 ± 21 | — |
| FMLP 10−6 mol/L | 127 ± 14 | — |
| RANTES 20 ng | 150 ± 10 | (*) |
| RANTES 10 ng | 142 ± 11 | (**) |
| RANTES 5 ng | 80 ± 16 | — |
| MCP-1 20 ng | 110 ± 15 | .05 |
| MCP-1 10 ng | 88 ± 10 | .01 |
| MCP-1 5 ng | 49 ± 13 | — |
| Treatment . | No. of Cells in 200 mm2 . | P< . |
|---|---|---|
| PBS (control) | 32 ± 12 | — |
| LPS 10 ng | 193 ± 21 | — |
| FMLP 10−6 mol/L | 127 ± 14 | — |
| RANTES 20 ng | 150 ± 10 | (*) |
| RANTES 10 ng | 142 ± 11 | (**) |
| RANTES 5 ng | 80 ± 16 | — |
| MCP-1 20 ng | 110 ± 15 | .05 |
| MCP-1 10 ng | 88 ± 10 | .01 |
| MCP-1 5 ng | 49 ± 13 | — |
Number of basophilic cells responding to exogenous human RANTES and MCP-1 at various doses in 50 μL intradermal injection in the rat. The sections were stained with Toluidine blue at 0.1% and counted in an optic field using a grating size 5 × 5 mm under an optic microscope × 40. This representative experiment was found to be reproducible and has been performed at least three times.