Table 2. Labeling of Highly Enriched... | American Society of Hematology

Table 2.

Labeling of Highly Enriched Populations of Primitive and Mature Hemopoietic Stem Cells and Progenitor Cells

¹²⁵I-Cytokine	Primitive HSC* Rh^lolin⁻Sca-1⁺c-kit⁺			Mature HSC* Rh^hilin⁻Sca-1⁺c-kit⁺			Progenitors* lin⁻Sca-1⁻c-kit⁺
	% Cells Labeled	Av Grains/Labeled Cell^‡	Receptors/Labeled Cell	% Cells Labeled	Av Grains/Labeled Cell^‡	Receptors/Labeled Cell	% Cells Labeled	Av Grains/Labeled Cell^‡	Receptors/Labeled Cell
rr′ SCF	^†			^†			17^†	21 ± 29	960
rh M-CSF	3	30 ± 29	90	0	0	0	0	0	0
rm IL-3	65	16 ± 12	80	69	24 ± 15	110	93	24 ± 24	110
rm GM-CSF	0	0	0	3	6 ± 4	190	55	18 ± 14	560
nm G-CSF	79	22 ± 21	60	73	30 ± 22	80	93	10 ± 11	30
rm IL-6	13	10 ± 2	170	18	10 ± 5	170	22	24 ± 14	400
rh LIF	0	0	0	0	0	0	1	5 ± 1	30
rh IL-1α	56	5 ± 2	nd	64	17 ± 11	nd	43	6 ± 3	nd

¹²⁵I-Cytokine	Primitive HSC* Rh^lolin⁻Sca-1⁺c-kit⁺			Mature HSC* Rh^hilin⁻Sca-1⁺c-kit⁺			Progenitors* lin⁻Sca-1⁻c-kit⁺
	% Cells Labeled	Av Grains/Labeled Cell^‡	Receptors/Labeled Cell	% Cells Labeled	Av Grains/Labeled Cell^‡	Receptors/Labeled Cell	% Cells Labeled	Av Grains/Labeled Cell^‡	Receptors/Labeled Cell
rr′ SCF	^†			^†			17^†	21 ± 29	960
rh M-CSF	3	30 ± 29	90	0	0	0	0	0	0
rm IL-3	65	16 ± 12	80	69	24 ± 15	110	93	24 ± 24	110
rm GM-CSF	0	0	0	3	6 ± 4	190	55	18 ± 14	560
nm G-CSF	79	22 ± 21	60	73	30 ± 22	80	93	10 ± 11	30
rm IL-6	13	10 ± 2	170	18	10 ± 5	170	22	24 ± 14	400
rh LIF	0	0	0	0	0	0	1	5 ± 1	30
rh IL-1α	56	5 ± 2	nd	64	17 ± 11	nd	43	6 ± 3	nd

All labeling was completely inhibited in the presence of a 100-fold excess of the appropriate unlabeled cytokine.

Abbreviation: nd, not determined because Scatchard analysis was not performed for rh IL-1α.

Sca-1 is identical to Ly6A/E.

†

Anti c-kit antibody (ACK2) inhibited binding of SCF to c-kit–positive cells. This effect could be partially reversed by acid treating the cells before performing SCF binding.

‡

Mean grain counts ± standard deviations of labeled cells.

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