Table 5. Detection of Donor Progenitor... | American Society of Hematology

Table 5.

Detection of Donor Progenitor Cells in the Bone Marrow of PBPC Recipients 2 Years After Transplant With Cells From the Blood of Donors Treated With FL, G-CSF, or FL + G-CSF

Mouse No.^5-150	FL
	PDGFR⁺^5-151		Y⁺^5-152	% Y⁺ρ	G-CSF
	No.	%			PDGFR⁺^5-151		Y⁺^5-152	% Y⁺ρ	G-CSF + FL
					No.	%			PDGFR⁺^5-151		Y⁺^5-152	% Y⁺ρ
									No.	%
1	15/44	34	0/15	0	32/44	73	29/32	91	10/27	37	8/10	80
2	13/44	30	0/13	0	22/44	50	17/22	77	43/44	98	40/43	93
3	19/44	43	0/19	0	13/44	30	4/13	31	20/44	46	20/20	100
4	16/44	36	0/16	0	40/44	91	39/40	98	18/42	43	10/18	56
5	38/44	86	2/38	5.3	22/44	50	15/22	68
6	25/44	57	0/25	0	38/44	86	37/38	97
Mean^5-155	48			0.9	63			77	56			82

Mouse No.^5-150	FL
	PDGFR⁺^5-151		Y⁺^5-152	% Y⁺ρ	G-CSF
	No.	%			PDGFR⁺^5-151		Y⁺^5-152	% Y⁺ρ	G-CSF + FL
					No.	%			PDGFR⁺^5-151		Y⁺^5-152	% Y⁺ρ
									No.	%
1	15/44	34	0/15	0	32/44	73	29/32	91	10/27	37	8/10	80
2	13/44	30	0/13	0	22/44	50	17/22	77	43/44	98	40/43	93
3	19/44	43	0/19	0	13/44	30	4/13	31	20/44	46	20/20	100
4	16/44	36	0/16	0	40/44	91	39/40	98	18/42	43	10/18	56
5	38/44	86	2/38	5.3	22/44	50	15/22	68
6	25/44	57	0/25	0	38/44	86	37/38	97
Mean^5-155	48			0.9	63			77	56			82

F5-150

Up to 6 mice that had received a PBPC graft mobilized with FL, G-CSF, or G-CSF + FL were assessed individually.

F5-151

Forty-four colonies grown from the bone marrow of each mouse were picked from the plates, and only samples that contained sufficient PDGFR to yield a positive signal were assessed for the Y-specific sequence.

F5-152

Number of colonies that were assessable in which Y-specific sequences were found.

ρ Percentage of progenitor cell colonies that were donor (male)-derived.

F5-155

Mean percentage of assessable colonies or donor (male) progenitor cells.

View Large

This Feature Is Available To Subscribers Only