Pulmonary Disease in Transplanted X-CGD Mice After Respiratory Challenge With A fumigatus Conidia
| Transplant Group . | N . | % NBT+ PMNs . | A fumigatus Lung Disease* . |
|---|---|---|---|
| None | 16 | 0 | 16/16 |
| MSCV-91Neo-transduced X-CGD BM† | |||
| MSCV-h91Neo (2 × 105 cfu/mL) | 4 | 1-2 | 4/4 |
| MSCV-m91Neo (1 × 106 cfu/mL) | 9 | 53-82 | 0/9 |
| Wild-type X-CGD BM‡ | |||
| Group I | 4 | 2-3 | 4/4 |
| Group II | 6 | 5-8 | 0/6 |
| Group III | 3 | 10-20 | 0/3 |
| Group IV | 4 | 20-35 | 0/4 |
| Group V | 4 | 90-98 | 0/4 |
| Transplant Group . | N . | % NBT+ PMNs . | A fumigatus Lung Disease* . |
|---|---|---|---|
| None | 16 | 0 | 16/16 |
| MSCV-91Neo-transduced X-CGD BM† | |||
| MSCV-h91Neo (2 × 105 cfu/mL) | 4 | 1-2 | 4/4 |
| MSCV-m91Neo (1 × 106 cfu/mL) | 9 | 53-82 | 0/9 |
| Wild-type X-CGD BM‡ | |||
| Group I | 4 | 2-3 | 4/4 |
| Group II | 6 | 5-8 | 0/6 |
| Group III | 3 | 10-20 | 0/3 |
| Group IV | 4 | 20-35 | 0/4 |
| Group V | 4 | 90-98 | 0/4 |
X-CGD mice were transplanted with either mixtures of wild-type and X-CGD BM or X-CGD BM transduced with MSCV-91Neo retroviruses. At the indicated times after transplant, X-CGD mice (along with control X-CGD mice that did not receive BM transplants) received a respiratory challenge with A fumigatus conidia, as described in Materials and Methods. All mice survived, and at 17 to 21 days after challenge, lungs were removed for histologic examination. The percentage of circulating NBT+ neutrophils was determined within 4 days before A fumigatus challenge, which also was similar to the percentage observed at time of sacrifice.
Granulomas, purulent bronchopneumonia, and/or abscesses.
Mice were challenged with 150 to 500 A fumigatus conidia 10 to 14 weeks after transplant.
Mice were challenged with 150 A fumigatus conidia 5 to 8 weeks after transplant, except for group V, which received 1 × 106 conidia.