Plasma cytokine/chemokine levels (mean ± SEM, pg/mL)
| Cytokine/chemokine . | Before LPS injection . | After LPS injection . | ||||
|---|---|---|---|---|---|---|
| WT, n = 7 . | Tg-sP-selectin, n = 8 . | P . | WT, n = 5 . | Tg-sP-selectin, n = 7 . | P . | |
| IL-1α | 26 ± 10 | 20 ± 6 | .62 | 79 ± 20 | 65 ± 5 | .43 |
| IL-1β | 39 ± 5 | 30 ± 6 | .30 | 347 ± 46 | 262 ± 26 | .12 |
| IL-2 | 22 ± 4 | 19 ± 3 | .67 | 43 ± 7 | 58 ± 11 | .29 |
| IL-3 | 6 ± 1 | 5 ± 1 | .40 | 24 ± 3 | 23 ± 4 | .73 |
| IL-4 | 15 ± 2 | 12 ± 1 | .16 | 23 ± 2 | 21 ± 2 | .54 |
| IL-5 | 27 ± 6 | 27 ± 2 | .95 | 102 ± 17 | 104 ± 14 | .92 |
| IL-6 | 20 ± 5 | 16 ± 2 | .37 | 15 648 ± 145 | 14 558 ± 368 | .06 |
| IL-9 | 555 ± 20 | 46 ± 10 | .67 | 159 ± 14 | 153 ± 23 | .84 |
| IL-10 | 113 ± 19 | 96 ± 8 | .40 | 3 007 ± 995 | 1 667 ± 376 | .18 |
| IL-12 (p40) | 424 ± 40 | 379 ± 36 | .41 | 6 823 ± 1322 | 6 557 ± 481 | .82 |
| IL-12 (p70) | 155 ± 35 | 123 ± 24 | .45 | 1 189 ± 69 | 1 113 ± 57 | .41 |
| IL-13 | 104 ± 17 | 199 ± 94 | .36 | 385 ± 21 | 438 ± 50 | .42 |
| IL-17 | 67 ± 10 | 59 ± 8 | .54 | 225 ± 37 | 186 ± 23 | .37 |
| Eotaxin | 1054 ± 287 | 1423 ± 270 | .36 | 8 592 ± 417 | 8 390 ± 373 | .73 |
| G-CSF | 148 ± 66 | 121 ± 25 | .69 | 50 528 ± 4747 | 49 630 ± 3439 | .88 |
| GM-CSF | 47 ± 6 | 41 ± 10 | .64 | 188 ± 31 | 179 ± 8 | .75 |
| IFN-γ | 38 ± 11 | 32 ± 6 | .60 | 93 ± 11 | 96 ± 14 | .90 |
| CXCL1 | 102 ± 16 | 128 ± 29 | .48 | 22 949 ± 1245 | 23 207 ± 1612 | .91 |
| MCP-1 | 313 ± 47 | 270 ± 35 | .47 | 37 767 ± 2331 | 35 583 ± 3203 | .62 |
| MIP-1α | 7 ± 1 | 6 ± 1 | .29 | 2 416 ± 801 | 1 613 ± 192 | .28 |
| MIP-1β | 37 ± 8 | 33 ± 6 | .70 | 19 954 ± 2801 | 18 613 ± 2135 | .71 |
| RANTES | 23 ± 4 | 32 ± 12 | .55 | 4 834 ± 1255 | 4 615 ± 286 | .85 |
| TNF-α | 490 ± 82 | 392 ± 53 | .32 | 1 201 ± 223 | 850 ± 59 | .11 |
| Cytokine/chemokine . | Before LPS injection . | After LPS injection . | ||||
|---|---|---|---|---|---|---|
| WT, n = 7 . | Tg-sP-selectin, n = 8 . | P . | WT, n = 5 . | Tg-sP-selectin, n = 7 . | P . | |
| IL-1α | 26 ± 10 | 20 ± 6 | .62 | 79 ± 20 | 65 ± 5 | .43 |
| IL-1β | 39 ± 5 | 30 ± 6 | .30 | 347 ± 46 | 262 ± 26 | .12 |
| IL-2 | 22 ± 4 | 19 ± 3 | .67 | 43 ± 7 | 58 ± 11 | .29 |
| IL-3 | 6 ± 1 | 5 ± 1 | .40 | 24 ± 3 | 23 ± 4 | .73 |
| IL-4 | 15 ± 2 | 12 ± 1 | .16 | 23 ± 2 | 21 ± 2 | .54 |
| IL-5 | 27 ± 6 | 27 ± 2 | .95 | 102 ± 17 | 104 ± 14 | .92 |
| IL-6 | 20 ± 5 | 16 ± 2 | .37 | 15 648 ± 145 | 14 558 ± 368 | .06 |
| IL-9 | 555 ± 20 | 46 ± 10 | .67 | 159 ± 14 | 153 ± 23 | .84 |
| IL-10 | 113 ± 19 | 96 ± 8 | .40 | 3 007 ± 995 | 1 667 ± 376 | .18 |
| IL-12 (p40) | 424 ± 40 | 379 ± 36 | .41 | 6 823 ± 1322 | 6 557 ± 481 | .82 |
| IL-12 (p70) | 155 ± 35 | 123 ± 24 | .45 | 1 189 ± 69 | 1 113 ± 57 | .41 |
| IL-13 | 104 ± 17 | 199 ± 94 | .36 | 385 ± 21 | 438 ± 50 | .42 |
| IL-17 | 67 ± 10 | 59 ± 8 | .54 | 225 ± 37 | 186 ± 23 | .37 |
| Eotaxin | 1054 ± 287 | 1423 ± 270 | .36 | 8 592 ± 417 | 8 390 ± 373 | .73 |
| G-CSF | 148 ± 66 | 121 ± 25 | .69 | 50 528 ± 4747 | 49 630 ± 3439 | .88 |
| GM-CSF | 47 ± 6 | 41 ± 10 | .64 | 188 ± 31 | 179 ± 8 | .75 |
| IFN-γ | 38 ± 11 | 32 ± 6 | .60 | 93 ± 11 | 96 ± 14 | .90 |
| CXCL1 | 102 ± 16 | 128 ± 29 | .48 | 22 949 ± 1245 | 23 207 ± 1612 | .91 |
| MCP-1 | 313 ± 47 | 270 ± 35 | .47 | 37 767 ± 2331 | 35 583 ± 3203 | .62 |
| MIP-1α | 7 ± 1 | 6 ± 1 | .29 | 2 416 ± 801 | 1 613 ± 192 | .28 |
| MIP-1β | 37 ± 8 | 33 ± 6 | .70 | 19 954 ± 2801 | 18 613 ± 2135 | .71 |
| RANTES | 23 ± 4 | 32 ± 12 | .55 | 4 834 ± 1255 | 4 615 ± 286 | .85 |
| TNF-α | 490 ± 82 | 392 ± 53 | .32 | 1 201 ± 223 | 850 ± 59 | .11 |
P value determined by the 2-tailed Student t test. Comparisons are between WT and Tg-sP-selectin mice in each experimental group (before or after LPS injection).
G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN, interferon; LPS, lipopolysaccharide; MCP, monocyte chemoattractant protein; MIP, macrophage inflammatory protein; TNF, tumor necrosis factor.