High-resolution HLA class II typing of recipient and CBUs
| Patient no. . | . | HLA-A . | HLA-B . | HLA-C . | HLA-DRB1* . | HLA-DQ . | HLA-DP . |
|---|---|---|---|---|---|---|---|
| 1 | Recipient | 03:01, 32:01 | 27:05, 40:01 | 02:02, 03:04 | 08:01, 13:01 | 04:02, 06:03 | 04:01, 02:02 |
| 1 | PD-CBU | 03:01, 02:01 | –, 40:01 | –, 03:04 | 08:01, 13:01 | 04:02, 0603 | 04:01, 14:01 |
| 1 | NE-CBU | 03:01, 23:01 | 40:02, 40:01, | 02:02, 03:04 | 08:01, 13:01 | 04:02, 06xx | 04:01, 04:02 |
| 2 | Recipient | 02:05, 68:02 | 14:02, 50:01 | 06:02, 08:02 | 04:01, 04:08 | 03:01, 03:02 | 04:01, 04:02 |
| 2 | PD-CBU | 02:01, 68:02 | 14:02, 44:02 | 05:01, 08:02 | 04:01, 13:03 | 03:01,– | 04:01,– |
| 2 | NE-CBU | 02:01, 68:02 | 14:02, 44:xx | 05:01, 08:02 | 04:01, 01:02 | 03:01, 05:01 | 04:01, 13:01 |
| 3 | Recipient | 23:01, 24:03 | 38:01, 57:03 | 07:01, 12:03 | 03:02, 13:01 | 02:03, 06:03 | 02:01, 01:01 |
| 3 | PD-CBU | 24:02, 26:01 | 38:01, 57:01 | 06:02, 12:03 | 07:01, 13:01 | 03:03, 06;03 | –, 04:01 |
| 3 | NE-CBU | 24;02, 26:01 | 38:01, 57:01 | 06:02, 12:03 | 07:01, 13:01 | 03:03, 06:03 | 02:01, 04:01 |
| 4 | Recipient | 02:01, 32:01 | 15:01, 37:01 | 03:04, 06:02 | 03:01, 10:01 | 02:01, 05:01 | 01:01, 0201 |
| 4 | PD-CBU | 02:01, 25:01 | 15:01, 37:01 | 03:03, 06:02 | 15:01, 10:01 | 06:02, 05:01 | 04:01,– |
| 4 | NE-CBU | 01:01, 02:01 | 15:01, 37:01 | 03:03, 06:02 | 08:01, 10:01 | 04:02, 05:01 | 04:01, 02:01 |
| 5 | Recipient | 01:01, 24:02 | 15:01, 44:02 | 01:02,– | 01:01, 16:01 | 05:01, 05:02 | 02:01, 04:02 |
| 5 | PD-CBU | 01:01, 24:02 | 35:01, 44:05 | 02:02, 04:01 | 01:01, 16:01 | 05:01, 05:02 | 01:01, 16:01 |
| 5 | NE-CBU | 01:01, 24:02 | 15:01, 44:03 | 03:03, 07:01 | 01:01, 07:01 | 05:01, 02:02 | 10:01, 15:01 |
| 6 | Recipient | 25:01, 31:01 | 49:01, 57:01 | 06:02, 07:01 | 01:01, 08:01 | 04:02, 05:04 | 04:01, 16:01 |
| 6 | PD-CBU | 11:01, 31:01 | 39:06, 49:01 | 07:01, 07:02 | 01:01, 08:01 | 04:02, 05:04 | 03:01, 04:01 |
| 6 | NE-CBU | 26:01, 31:01 | 49:01, 15:01 | 03:03, 07:01 | 01:01, 08:01 | 04:02, 05:04 | 02:01, 13:01 |
| 7 | Recipient | 01:01, 31:01 | 44:02, 57:01 | 02:02, 06:02 | 04:04, 08:06 | 03:02, 05:01 | ND |
| 7 | PD-CBU | 01:01, 31:01 | 40:01, 57:01 | 03:04, 06:02 | 04:04, 07:01 | 03:02, 03:03 | 04:01,– |
| 7 | NE-CBU | 01:01, 31:01 | 40:01, 57:01 | 03:04, 06:02 | 04:04, 07:01 | 03:02, 03:03 | 04:01, 06:01 |
| 8 | Recipient | 03:01, 68:01 | 53:01, 57:03 | 06:02, 07:01 | 09:01, 14:54 | 02:02, 0:503 | 17:01,– |
| 8 | PD-CBU | 03:01, 68:02 | 53:01, 57:03 | 04:01, 07:18 | 13:02, 15:03 | 06:09, 06:02 | 02:01,– |
| 8 | NE-CBU | 03:02, 68:02 | 53:01, 57:01 | 04:01, 06:02 | 07:01, 15:01 | 03:03, 06:02 | 04:01,– |
| 9 | Recipient | 01:01, 31:01 | 37:01, 40:01 | 03:04, 06:02 | 01:01, 13:03 | 03:01, 05:01 | 04:01,– |
| 9 | PD-CBU | 01:01, 31:01 | 37:01, 40:01 | 03:04, 06:02 | 01:01, 10:01 | 05:01, 05:01 | 02:01, 04:02 |
| 9 | NE-CBU | 01:01, 31:01 | 37:01, 40:01 | 03:04, 06:02 | 04:04, 15:01 | 03:02, 06:02 | 04:01,– |
| 10 | Recipient | 31:01, 33:01 | 07:02, 14:02 | 07:02, 08:02 | 04:03, 15:01 | 03:02, 06:02 | 03:01, 04:01 |
| 10 | PD-CBU | 03:01, 33:01 | 07:xx, 14:02 | 07:02, 08:02 | 01:02, 15:01 | 05:01, 06:02 | 02:01, 04:01 |
| 10 | NE-CBU | 31:01, 33:01 | 07:02, 14:02 | 07:02, 08:02 | 11:04, 15:01 | 03:01, 06:02 | 02:01, 04:01 |
| 11 | Recipient | 01:01, 11:01 | 07:02, 44:02 | 07:02, 07:04 | 04:04, 11:01 | 03:01, 03:02 | 03:01,– |
| 11 | PD-CBU | 01:01, 01:xx | 07:02, 44:02 | 07:02, 05:01 | 04:04, 11:01 | 03:01, 03:02 | 04:01,– |
| 11 | NE-CBU | 01:01, 29:02 | 07:02, 44:03 | 04:01, 07:01 | 15:01, 11:01 | 03:01, 06:02 | 04:01,– |
| Patient no. . | . | HLA-A . | HLA-B . | HLA-C . | HLA-DRB1* . | HLA-DQ . | HLA-DP . |
|---|---|---|---|---|---|---|---|
| 1 | Recipient | 03:01, 32:01 | 27:05, 40:01 | 02:02, 03:04 | 08:01, 13:01 | 04:02, 06:03 | 04:01, 02:02 |
| 1 | PD-CBU | 03:01, 02:01 | –, 40:01 | –, 03:04 | 08:01, 13:01 | 04:02, 0603 | 04:01, 14:01 |
| 1 | NE-CBU | 03:01, 23:01 | 40:02, 40:01, | 02:02, 03:04 | 08:01, 13:01 | 04:02, 06xx | 04:01, 04:02 |
| 2 | Recipient | 02:05, 68:02 | 14:02, 50:01 | 06:02, 08:02 | 04:01, 04:08 | 03:01, 03:02 | 04:01, 04:02 |
| 2 | PD-CBU | 02:01, 68:02 | 14:02, 44:02 | 05:01, 08:02 | 04:01, 13:03 | 03:01,– | 04:01,– |
| 2 | NE-CBU | 02:01, 68:02 | 14:02, 44:xx | 05:01, 08:02 | 04:01, 01:02 | 03:01, 05:01 | 04:01, 13:01 |
| 3 | Recipient | 23:01, 24:03 | 38:01, 57:03 | 07:01, 12:03 | 03:02, 13:01 | 02:03, 06:03 | 02:01, 01:01 |
| 3 | PD-CBU | 24:02, 26:01 | 38:01, 57:01 | 06:02, 12:03 | 07:01, 13:01 | 03:03, 06;03 | –, 04:01 |
| 3 | NE-CBU | 24;02, 26:01 | 38:01, 57:01 | 06:02, 12:03 | 07:01, 13:01 | 03:03, 06:03 | 02:01, 04:01 |
| 4 | Recipient | 02:01, 32:01 | 15:01, 37:01 | 03:04, 06:02 | 03:01, 10:01 | 02:01, 05:01 | 01:01, 0201 |
| 4 | PD-CBU | 02:01, 25:01 | 15:01, 37:01 | 03:03, 06:02 | 15:01, 10:01 | 06:02, 05:01 | 04:01,– |
| 4 | NE-CBU | 01:01, 02:01 | 15:01, 37:01 | 03:03, 06:02 | 08:01, 10:01 | 04:02, 05:01 | 04:01, 02:01 |
| 5 | Recipient | 01:01, 24:02 | 15:01, 44:02 | 01:02,– | 01:01, 16:01 | 05:01, 05:02 | 02:01, 04:02 |
| 5 | PD-CBU | 01:01, 24:02 | 35:01, 44:05 | 02:02, 04:01 | 01:01, 16:01 | 05:01, 05:02 | 01:01, 16:01 |
| 5 | NE-CBU | 01:01, 24:02 | 15:01, 44:03 | 03:03, 07:01 | 01:01, 07:01 | 05:01, 02:02 | 10:01, 15:01 |
| 6 | Recipient | 25:01, 31:01 | 49:01, 57:01 | 06:02, 07:01 | 01:01, 08:01 | 04:02, 05:04 | 04:01, 16:01 |
| 6 | PD-CBU | 11:01, 31:01 | 39:06, 49:01 | 07:01, 07:02 | 01:01, 08:01 | 04:02, 05:04 | 03:01, 04:01 |
| 6 | NE-CBU | 26:01, 31:01 | 49:01, 15:01 | 03:03, 07:01 | 01:01, 08:01 | 04:02, 05:04 | 02:01, 13:01 |
| 7 | Recipient | 01:01, 31:01 | 44:02, 57:01 | 02:02, 06:02 | 04:04, 08:06 | 03:02, 05:01 | ND |
| 7 | PD-CBU | 01:01, 31:01 | 40:01, 57:01 | 03:04, 06:02 | 04:04, 07:01 | 03:02, 03:03 | 04:01,– |
| 7 | NE-CBU | 01:01, 31:01 | 40:01, 57:01 | 03:04, 06:02 | 04:04, 07:01 | 03:02, 03:03 | 04:01, 06:01 |
| 8 | Recipient | 03:01, 68:01 | 53:01, 57:03 | 06:02, 07:01 | 09:01, 14:54 | 02:02, 0:503 | 17:01,– |
| 8 | PD-CBU | 03:01, 68:02 | 53:01, 57:03 | 04:01, 07:18 | 13:02, 15:03 | 06:09, 06:02 | 02:01,– |
| 8 | NE-CBU | 03:02, 68:02 | 53:01, 57:01 | 04:01, 06:02 | 07:01, 15:01 | 03:03, 06:02 | 04:01,– |
| 9 | Recipient | 01:01, 31:01 | 37:01, 40:01 | 03:04, 06:02 | 01:01, 13:03 | 03:01, 05:01 | 04:01,– |
| 9 | PD-CBU | 01:01, 31:01 | 37:01, 40:01 | 03:04, 06:02 | 01:01, 10:01 | 05:01, 05:01 | 02:01, 04:02 |
| 9 | NE-CBU | 01:01, 31:01 | 37:01, 40:01 | 03:04, 06:02 | 04:04, 15:01 | 03:02, 06:02 | 04:01,– |
| 10 | Recipient | 31:01, 33:01 | 07:02, 14:02 | 07:02, 08:02 | 04:03, 15:01 | 03:02, 06:02 | 03:01, 04:01 |
| 10 | PD-CBU | 03:01, 33:01 | 07:xx, 14:02 | 07:02, 08:02 | 01:02, 15:01 | 05:01, 06:02 | 02:01, 04:01 |
| 10 | NE-CBU | 31:01, 33:01 | 07:02, 14:02 | 07:02, 08:02 | 11:04, 15:01 | 03:01, 06:02 | 02:01, 04:01 |
| 11 | Recipient | 01:01, 11:01 | 07:02, 44:02 | 07:02, 07:04 | 04:04, 11:01 | 03:01, 03:02 | 03:01,– |
| 11 | PD-CBU | 01:01, 01:xx | 07:02, 44:02 | 07:02, 05:01 | 04:04, 11:01 | 03:01, 03:02 | 04:01,– |
| 11 | NE-CBU | 01:01, 29:02 | 07:02, 44:03 | 04:01, 07:01 | 15:01, 11:01 | 03:01, 06:02 | 04:01,– |
HLA mismatches between both recipient and NE-CBU vs PD-CBU are depicted in bold.
ND, no data available; –, no second allele identified; xx, allele digits not specified.
In the case of a specific mismatched DRB1 allele (between UCBs), we also tested the mismatching paralogue allele (selection based on frequency prediction); see Table 4.