GAPDH Cys and His modifications in stored RBC membrane and vesicle
| . | C152* . | C156* . | C247* . | H165* . | H179* . | m/z [Theor.] . | m/z [Exp.] . | Δ ppm . | Score† . |
|---|---|---|---|---|---|---|---|---|---|
| IISNASCTTNCLAPLAK | CM | CM | 917.4640 | 917.4630 | 1.1 | 108.2 | |||
| Diox. | CM | 904.9482 | 904.9445 | 4.1 | 33.5 | ||||
| CM | Diox. | 904.9482 | 904.9448 | 3.8 | 40.9 | ||||
| DHA | CM | 871.9595 | 871.9570 | 2.9 | 86.0 | ||||
| CM | DHA | 871.9595 | 871.9559 | 4.1 | 52.9 | ||||
| Diox. | DHA | 859.4436 | 859.4316 | 13.9 | 53.9 | ||||
| DHA | Diox. | 859.4436 | 859.4318 | 13.7 | 51.5 | ||||
| Sulfur dioxide | CM | 920.9265 | 920.9309 | 4.8 | 91.5 | ||||
| VPTANVSVVDLTCR | CM | 765.9014 | 765.9017 | 0.4 | 92.7 | ||||
| Diox. | 753.3856 | 753.3828 | 3.7 | 60.2 | |||||
| DHA | 720.3968 | 720.3950 | 2.5 | 68.1 | |||||
| VIHDNFGIVEGLMTTVHAITATQK | Oxidation | Unmodified | 871.1159 | 871.1215 | 6.4 | 57.7 | |||
| Unmodified | Oxidation | 876.4475‡ | 876.4535 | 6.8 | 51.2 |
| . | C152* . | C156* . | C247* . | H165* . | H179* . | m/z [Theor.] . | m/z [Exp.] . | Δ ppm . | Score† . |
|---|---|---|---|---|---|---|---|---|---|
| IISNASCTTNCLAPLAK | CM | CM | 917.4640 | 917.4630 | 1.1 | 108.2 | |||
| Diox. | CM | 904.9482 | 904.9445 | 4.1 | 33.5 | ||||
| CM | Diox. | 904.9482 | 904.9448 | 3.8 | 40.9 | ||||
| DHA | CM | 871.9595 | 871.9570 | 2.9 | 86.0 | ||||
| CM | DHA | 871.9595 | 871.9559 | 4.1 | 52.9 | ||||
| Diox. | DHA | 859.4436 | 859.4316 | 13.9 | 53.9 | ||||
| DHA | Diox. | 859.4436 | 859.4318 | 13.7 | 51.5 | ||||
| Sulfur dioxide | CM | 920.9265 | 920.9309 | 4.8 | 91.5 | ||||
| VPTANVSVVDLTCR | CM | 765.9014 | 765.9017 | 0.4 | 92.7 | ||||
| Diox. | 753.3856 | 753.3828 | 3.7 | 60.2 | |||||
| DHA | 720.3968 | 720.3950 | 2.5 | 68.1 | |||||
| VIHDNFGIVEGLMTTVHAITATQK | Oxidation | Unmodified | 871.1159 | 871.1215 | 6.4 | 57.7 | |||
| Unmodified | Oxidation | 876.4475‡ | 876.4535 | 6.8 | 51.2 |
Underlined letters represent site of modification.
CM, carbamidomethyl; Diox., dioxidation; m/z [Exp.], experimental mass-to-charge ratio; m/z [Theor.], theoretical mass-to-charge ratio; ppm, parts per million.
Tentative assignment based on Mascot search against human SwissProt database.
Highest Mascot score observed for the given modified peptide among all samples.
Both M175 sulfoxide and 2-oxo-H179 observed in the reported spectral match.