Affinity constants derived from Biacore and flow cytometric studies
| Method . | Ligand . | Receptor . | KD values (mol/L) . | Mean KD (mol/L) . |
|---|---|---|---|---|
| Biacore | Quinine | mAb 314.1 | 2.8, 2.5, 2.7 (× 10−8)* | 2.7 × 10−8 |
| Biacore | Quinine | mAb 314.3 | 2.5, 3.6, 3.6 (× 10−8)* | 3.2 × 10−8 |
| Biacore | GPIIb/IIIa - Qn | mAb 314.1 | 8.6, 8.2, 17 (× 10−6)* | 1.1 × 10−5 |
| Biacore | GPIIb/IIIa + Qn | mAb 314.1 | 2.9, 2.9, 1.2, 1.7 (× 10−6)* | 2.2 × 10−6 |
| Flow cytometry | 314.1 Fab + Qn | Platelet GPIIb | 1.9, 2.2, 1.8, 1.7 (× 10−5) | 1.9 × 10−5 |
| Flow cytometry | 314.1 IgG + Qn | Platelet GPIIb | 1.4, 1.4, 1.4, 1.7, 1.6 (× 10−10) | 1.5 × 10−10 |
| Method . | Ligand . | Receptor . | KD values (mol/L) . | Mean KD (mol/L) . |
|---|---|---|---|---|
| Biacore | Quinine | mAb 314.1 | 2.8, 2.5, 2.7 (× 10−8)* | 2.7 × 10−8 |
| Biacore | Quinine | mAb 314.3 | 2.5, 3.6, 3.6 (× 10−8)* | 3.2 × 10−8 |
| Biacore | GPIIb/IIIa - Qn | mAb 314.1 | 8.6, 8.2, 17 (× 10−6)* | 1.1 × 10−5 |
| Biacore | GPIIb/IIIa + Qn | mAb 314.1 | 2.9, 2.9, 1.2, 1.7 (× 10−6)* | 2.2 × 10−6 |
| Flow cytometry | 314.1 Fab + Qn | Platelet GPIIb | 1.9, 2.2, 1.8, 1.7 (× 10−5) | 1.9 × 10−5 |
| Flow cytometry | 314.1 IgG + Qn | Platelet GPIIb | 1.4, 1.4, 1.4, 1.7, 1.6 (× 10−10) | 1.5 × 10−10 |
Values shown are the means of replicate determinations performed with the same flow cell.