Summary of the hit-validation results
| Expressed target gene . | No. of depleted shRNAs . | Mean z score of depleted shRNAs . | No. of validated shRNAs . |
|---|---|---|---|
| BNIPL | 2 | −2.6 | 2 |
| DGAT2 | 2 | −2.4 | 1 |
| DUSP14 | 2 | −2.5 | 0 |
| MAP3K6 | 2 | −2.6 | 2 |
| ROCK1 | 3 | −2.4 | 2 |
| RPS13 | 2 | −2.9 | 2 |
| SF3A1 | 2 | −2.5 | 1 |
| SNX27 | 2 | −3.5 | 2 |
| STK3 | 2 | −2.4 | 2 |
| WDHD1 | 2 | −2.4 | 0 |
| Expressed target gene . | No. of depleted shRNAs . | Mean z score of depleted shRNAs . | No. of validated shRNAs . |
|---|---|---|---|
| BNIPL | 2 | −2.6 | 2 |
| DGAT2 | 2 | −2.4 | 1 |
| DUSP14 | 2 | −2.5 | 0 |
| MAP3K6 | 2 | −2.6 | 2 |
| ROCK1 | 3 | −2.4 | 2 |
| RPS13 | 2 | −2.9 | 2 |
| SF3A1 | 2 | −2.5 | 1 |
| SNX27 | 2 | −3.5 | 2 |
| STK3 | 2 | −2.4 | 2 |
| WDHD1 | 2 | −2.4 | 0 |
All expressed target genes were reanalyzed in single-transduction hit-validation experiments. The numbers of shRNAs depleted by a z score of <−2 in the primary screen are given with their mean z score. The last column shows the number of shRNAs that could successfully be validated in single-transduction experiments.