Immune cell populations in lymphoid and nonlymphoid organs from NKR-P1B-deficient mice
| Organ . | Cell populations . | Cell numbers . | |
|---|---|---|---|
| Nkrp1b+/+ . | Nkrp1b−/− . | ||
| Thymus (×106) | Total | 131.6 ± 27.3 | 118.5 ± 32 |
| CD4 SP | 9.9 ± 4.2 | 11.4 ± 3.4 | |
| CD8 SP | 4.63 ± 3.3 | 4.0 ± 1.1 | |
| CD4 CD8 DP | 112.3 ± 22 | 98.9 ± 27.7 | |
| NKT | 0.45 ± 0.22 | 0.5 ± 0.04 | |
| Spleen (×106) | Total | 87.1 ± 10.4 | 83.4 ± 15.4 |
| CD4 | 19.0 ± 2.8 | 18.2 ± 5.3 | |
| CD8 | 12.2 ± 1.3 | 11.3 ± 2.7 | |
| B | 45.1 ± 12.7 | 44.1 ± 14.6 | |
| NK | 3.99 ± 0.8 | 3.68 ± 1.3 | |
| NKT | 1.27 ± 0.24 | 1.29 ± 0.32 | |
| DC | 2.5 ± 0.7 | 2.7 ± 0.7 | |
| pDC | 0.17 ± 0.08 | 0.17 ± 0.06 | |
| Lungs (×105) | Total | 17.0 ± 3.4 | 18.4 ± 2.4 |
| NK | 0.60 ± 0.1 | 0.85 ± 0.3 | |
| NKT | 0.054 ± 0.01 | 0.072 ± 0.01 | |
| Liver (×105) | Total | 26.2 ± 8 | 25.0 ± 6.2 |
| NK | 1.9 ± 0.3 | 2.0 ± 0.4 | |
| NKT | 1.11 ± 0.4 | 1.13 ± 0.39 | |
| Organ . | Cell populations . | Cell numbers . | |
|---|---|---|---|
| Nkrp1b+/+ . | Nkrp1b−/− . | ||
| Thymus (×106) | Total | 131.6 ± 27.3 | 118.5 ± 32 |
| CD4 SP | 9.9 ± 4.2 | 11.4 ± 3.4 | |
| CD8 SP | 4.63 ± 3.3 | 4.0 ± 1.1 | |
| CD4 CD8 DP | 112.3 ± 22 | 98.9 ± 27.7 | |
| NKT | 0.45 ± 0.22 | 0.5 ± 0.04 | |
| Spleen (×106) | Total | 87.1 ± 10.4 | 83.4 ± 15.4 |
| CD4 | 19.0 ± 2.8 | 18.2 ± 5.3 | |
| CD8 | 12.2 ± 1.3 | 11.3 ± 2.7 | |
| B | 45.1 ± 12.7 | 44.1 ± 14.6 | |
| NK | 3.99 ± 0.8 | 3.68 ± 1.3 | |
| NKT | 1.27 ± 0.24 | 1.29 ± 0.32 | |
| DC | 2.5 ± 0.7 | 2.7 ± 0.7 | |
| pDC | 0.17 ± 0.08 | 0.17 ± 0.06 | |
| Lungs (×105) | Total | 17.0 ± 3.4 | 18.4 ± 2.4 |
| NK | 0.60 ± 0.1 | 0.85 ± 0.3 | |
| NKT | 0.054 ± 0.01 | 0.072 ± 0.01 | |
| Liver (×105) | Total | 26.2 ± 8 | 25.0 ± 6.2 |
| NK | 1.9 ± 0.3 | 2.0 ± 0.4 | |
| NKT | 1.11 ± 0.4 | 1.13 ± 0.39 | |
Single-cell suspensions from different organs were stained with antibodies for cell-specific markers and analyzed by flow cytometry. Values are presented as the mean ± SD from 6 to 7 mice of each genotype.
DC, dendritic cells; DP, double positive; SP, single positive; pDC, plasmacytoid dendritic cells.