Percentage of different CFU types generated by HSPCs grown in Epo, Tpo, and Gcsfa
Colony type . | Incidence of CFUs formed when plating cell fractions below in Epo, Tpo, and Gcsfa, % . | |
---|---|---|
Fractionated WKM . | Cd41:GFPmedium L+P cells . | |
BFU-E | 0.19 | 1.71 |
CFU-E | 1.15 | 12.22 |
CFU-T | 0.08 | 0.75 |
CFU-TE | 0.006 | 0.13 |
CFU-GM | 6.47 | 6.75 |
CFU-GMT | 0.04 | 0.28 |
CFU-GEMT | 0.004 | 0.08 |
Colony type . | Incidence of CFUs formed when plating cell fractions below in Epo, Tpo, and Gcsfa, % . | |
---|---|---|
Fractionated WKM . | Cd41:GFPmedium L+P cells . | |
BFU-E | 0.19 | 1.71 |
CFU-E | 1.15 | 12.22 |
CFU-T | 0.08 | 0.75 |
CFU-TE | 0.006 | 0.13 |
CFU-GM | 6.47 | 6.75 |
CFU-GMT | 0.04 | 0.28 |
CFU-GEMT | 0.004 | 0.08 |
Summary of CFU incidence when 100 000 cells (fractionated WKM or cd41:GFPmedium L+P cells) were plated in the presence of Epo, Tpo, and Gcsfa. These data are a summary of supplemental Figure 8. CFU-GEMT, CFU-granulocyte, erythroid, macrophage, thrombocyte.