Balb/c 3T3 cells (clone A31) were transfected with expression vectors encoding wild-type ARAF or ARAF containing the Q347_A348 deletion and the F351L substitution. Cells were seeded in 0.33% agar in complete growth medium. After 22 d in culture, the number of colonies with diameter >0.2 mm was counted over a 4-cm² area using a dissecting microscope. Means and standard deviations based on 2 wells are shown.