Kinetic parameters determined on FVIIIa inactivation by APC and PS in the presence of recombinant FV variants
| FV variants . | Rate constant: k, minutes−1 (-fold) . | |
|---|---|---|
| FVIIIa inactivation . | Cleavage at Arg336 . | |
| WT | 0.742 ± 0.034 (1) | 0.274 ± 0.034 (1) |
| W1920R | 0.239 ± 0.021 (0.32) | Not determined |
| R506Q | 0.390 ± 0.012 (0.53) | 0.110 ± 0.076 (0.40) |
| None | 0.246 ± 0.019 (0.33) | Not determined |
| FV variants . | Rate constant: k, minutes−1 (-fold) . | |
|---|---|---|
| FVIIIa inactivation . | Cleavage at Arg336 . | |
| WT | 0.742 ± 0.034 (1) | 0.274 ± 0.034 (1) |
| W1920R | 0.239 ± 0.021 (0.32) | Not determined |
| R506Q | 0.390 ± 0.012 (0.53) | 0.110 ± 0.076 (0.40) |
| None | 0.246 ± 0.019 (0.33) | Not determined |
Values were calculated by nonlinear least squares regression from the data shown in Figure 7A-B, using the single exponential decay.