Kinetic parameters determined on APC-catalyzed inactivation of recombinant FVa variants
| FVa variants . | PS, minutes−1 (-fold) . | |
|---|---|---|
| Plus . | Minus . | |
| WT | 1.07 ± 0.06 (1) | 0.230 ± 0.016 (1) |
| W1920R | 0.100 ± 0.001 (0.09) | 0.038 ± 0.008 (0.16) |
| R506Q | 0.417 ± 0.061 (0.39) | Not determined |
| FVa variants . | PS, minutes−1 (-fold) . | |
|---|---|---|
| Plus . | Minus . | |
| WT | 1.07 ± 0.06 (1) | 0.230 ± 0.016 (1) |
| W1920R | 0.100 ± 0.001 (0.09) | 0.038 ± 0.008 (0.16) |
| R506Q | 0.417 ± 0.061 (0.39) | Not determined |
Values were calculated by nonlinear least squares regression from the data shown in Figure 5A-B, using the single exponential decay.