Regions of mouse-human homology identified on the Ikzf1
| Conserved region . | Total no. bp of homology (no. of subregions) . | Percentage mouse-human homology in subregions (total) . | Mouse mm9 (Chr11) . | Human hg19 (Chr7) . | Length (bp) of fragment used for a transgenic cassette . | Tissue-specific DHS cluster in vicinity? . |
|---|---|---|---|---|---|---|
| J | 645 (1) | 96 | 11575935-11576581 | 50333380-50334026 | 647 | No |
| p* | 632 (2) | 97 | 11599576-11600207 | 50358080-50358643 | 442 | DHS-C4 |
| D† | 424 (7) | 93 | 11616550-11618284 | 50287655-50290663 (mm7 > hg18) | 1735 | DHS-C6 |
| E‡ | 258 (2) | 91 | 11625474-11626435 | 50418201-50419257 | 962 | DHS-C7 |
| F | 204 (1) | 86 | 11630128-11630533 | 50424272-50424659 | 406 | DHS-C8 |
| G | 161 (1) | 88 | 11633285-11633536 | 50427799-50427958 | 322 | No |
| H§ | 204 (3) | 95 | 11640927-11644360 | 50435678-50439913 | 3434 | DHS-C9 |
| I | 529 (1) | 96 | 11653307-11653987 | 50449525-50450219 | 681 | No |
| Conserved region . | Total no. bp of homology (no. of subregions) . | Percentage mouse-human homology in subregions (total) . | Mouse mm9 (Chr11) . | Human hg19 (Chr7) . | Length (bp) of fragment used for a transgenic cassette . | Tissue-specific DHS cluster in vicinity? . |
|---|---|---|---|---|---|---|
| J | 645 (1) | 96 | 11575935-11576581 | 50333380-50334026 | 647 | No |
| p* | 632 (2) | 97 | 11599576-11600207 | 50358080-50358643 | 442 | DHS-C4 |
| D† | 424 (7) | 93 | 11616550-11618284 | 50287655-50290663 (mm7 > hg18) | 1735 | DHS-C6 |
| E‡ | 258 (2) | 91 | 11625474-11626435 | 50418201-50419257 | 962 | DHS-C7 |
| F | 204 (1) | 86 | 11630128-11630533 | 50424272-50424659 | 406 | DHS-C8 |
| G | 161 (1) | 88 | 11633285-11633536 | 50427799-50427958 | 322 | No |
| H§ | 204 (3) | 95 | 11640927-11644360 | 50435678-50439913 | 3434 | DHS-C9 |
| I | 529 (1) | 96 | 11653307-11653987 | 50449525-50450219 | 681 | No |
Conserved regions listed in 5′ to 3′ order (top to bottom) according to their relative position on the Ikzf1 locus.
p was initially believed to be a promoter, based on the extent of cross-species conservation and the presence of TATA and CAAT box elements within the p sequence. However, transgenic cassettes using this fragment in the absence of untranslated exons 1a or 1b have failed to generate mice with lympho-myeloid specific reporter activity.
Region D consists of seven subregions (length, % homology): D1 (45 bp, 89%), D2 (52 bp, 96%), D3 (78 bp, 94%), D4 (38 bp, 94%), D5 (67 bp, 97%), D6 (102 bp, 86%), and D7 (35 bp, 94%) (supplemental Table 3).
Region E consists of two subregions (length, % homology): E1 (134 bp, 94%) and E2 (122 bp, 88%).
Region H consists of three subregions (length, % homology): H1 (115 bp, 93%), H2 (47 bp, 95%), and H3 (40 bp, 92%).