Numbers of EZH2 mutations detected using the different sequencing approaches
Mutations | Sanger sequencing and NGS | Additional mutations by NGS only | Total |
|---|---|---|---|
| K634E | 0 | 1 | 1 |
| V637A | 1 | 0 | 1 |
| Y646N | 18 | 18 | 36 |
| Y646F | 18 | 9 | 27 |
| Y646S | 8 | 4 | 12 |
| Y646H | 6 | 3 | 9 |
| Y646C | 0 | 3 | 3 |
| V679M | 0 | 1 | 1 |
| A682G | 7 | 2 | 9 |
| A692V | 5 | 2 | 7 |
| In total | 63 | 43 | 106 |
| Mean VAF (range) | 29.78% (4-61) | 9.71% (2-31) | 21.64% (2-61) |
Mutations | Sanger sequencing and NGS | Additional mutations by NGS only | Total |
|---|---|---|---|
| K634E | 0 | 1 | 1 |
| V637A | 1 | 0 | 1 |
| Y646N | 18 | 18 | 36 |
| Y646F | 18 | 9 | 27 |
| Y646S | 8 | 4 | 12 |
| Y646H | 6 | 3 | 9 |
| Y646C | 0 | 3 | 3 |
| V679M | 0 | 1 | 1 |
| A682G | 7 | 2 | 9 |
| A692V | 5 | 2 | 7 |
| In total | 63 | 43 | 106 |
| Mean VAF (range) | 29.78% (4-61) | 9.71% (2-31) | 21.64% (2-61) |