PLS3, Twist, KIR3DL2, and NKp46 mRNA expression in CD4+ isolated T cells from patients with SS (n = 81) and from healthy donors (n = 12)
| Markers . | T-plastin (PLS3) . | Twist . | KIR3DL2 . | NKp46 . |
|---|---|---|---|---|
| 2(−ΔΔCt) value (AU) in HuT-78 Sézary cell line | 128 | 316 | 13 150 | 7.3 |
| 2(−ΔΔCt) value (AU) in CD56+ NK/T malignant cell line | 0.01 | 0.12 | 0.5 | 181 |
| 2(−ΔΔCt) mean value (AU) ± SEM CD4+ T cells isolated from healthy donors | 2.4 ± 2.5 | 6.6 ± 8 | 22.5 ± 20.4 | 2.6 ± 2.5 |
| Threshold (95%) | 5 | 10 | 25 | 5 |
| 2(−ΔΔCt) mean value (AU) ± SEM (min-max) in CD4+-isolated T cells from CTCL patients | 726 ± 144 (0-6385) | 1511 ± 313 (0-1441) | 878 ± 102 (1-3746) | 7.4 ± 1.8 (0-95) |
| Fold/threshold | ×145 | ×150 | ×35 | ×1.5 |
| % positive patients/marker | 87 | 91 | 84 | 28 |
| Markers . | T-plastin (PLS3) . | Twist . | KIR3DL2 . | NKp46 . |
|---|---|---|---|---|
| 2(−ΔΔCt) value (AU) in HuT-78 Sézary cell line | 128 | 316 | 13 150 | 7.3 |
| 2(−ΔΔCt) value (AU) in CD56+ NK/T malignant cell line | 0.01 | 0.12 | 0.5 | 181 |
| 2(−ΔΔCt) mean value (AU) ± SEM CD4+ T cells isolated from healthy donors | 2.4 ± 2.5 | 6.6 ± 8 | 22.5 ± 20.4 | 2.6 ± 2.5 |
| Threshold (95%) | 5 | 10 | 25 | 5 |
| 2(−ΔΔCt) mean value (AU) ± SEM (min-max) in CD4+-isolated T cells from CTCL patients | 726 ± 144 (0-6385) | 1511 ± 313 (0-1441) | 878 ± 102 (1-3746) | 7.4 ± 1.8 (0-95) |
| Fold/threshold | ×145 | ×150 | ×35 | ×1.5 |
| % positive patients/marker | 87 | 91 | 84 | 28 |