Key crosslinks unique to the L260P tetramer
| Number . | Residue . | Crosslinked peptides* . | Spectrin domain . | MH+ . | Error (ppm) . | z . |
|---|---|---|---|---|---|---|
| 1 | 508-364 | AHI(EE)LR-HEAFE[K]STASWAER | α1C-β17B | 2497.222 | 3.04 | 4 |
| 2 | 265-466 | HEIDSY(DD)R-YQ[K]HQSLEAEVQTK | α4B-α1B | 2819.323 | 1.70 | 4 |
| 3 | 276-39 | FQSADETGQ[D]LVNANHEASDEVR-TEVLH[K]K | α4B-α2B | 3367.615 | 3.80 | 4 |
| 4 | 35-170 | T[E]VLHK-AE[K]LTLSHPSDAPQIQEM#K | α2B-α3B | 2846.472 | 1.97 | 4 |
| 5 | 35-167 | T[E]VLHK-ELCA[K]AEK | α2B-α3B | 1655.878 | 0.00 | 3 |
| 6 | 437-170 | DA(DD)LGK-AE[K]LTLSHPSDAPQIQEM#K | α1A-α3B | 2853.393 | 1.12 | 4 |
| Number . | Residue . | Crosslinked peptides* . | Spectrin domain . | MH+ . | Error (ppm) . | z . |
|---|---|---|---|---|---|---|
| 1 | 508-364 | AHI(EE)LR-HEAFE[K]STASWAER | α1C-β17B | 2497.222 | 3.04 | 4 |
| 2 | 265-466 | HEIDSY(DD)R-YQ[K]HQSLEAEVQTK | α4B-α1B | 2819.323 | 1.70 | 4 |
| 3 | 276-39 | FQSADETGQ[D]LVNANHEASDEVR-TEVLH[K]K | α4B-α2B | 3367.615 | 3.80 | 4 |
| 4 | 35-170 | T[E]VLHK-AE[K]LTLSHPSDAPQIQEM#K | α2B-α3B | 2846.472 | 1.97 | 4 |
| 5 | 35-167 | T[E]VLHK-ELCA[K]AEK | α2B-α3B | 1655.878 | 0.00 | 3 |
| 6 | 437-170 | DA(DD)LGK-AE[K]LTLSHPSDAPQIQEM#K | α1A-α3B | 2853.393 | 1.12 | 4 |
MH+, protonated molecular ion mass.
[X], crosslinked residue; (X), ambiguous assignment crosslinked residue; #, methionine oxidation.