5′-AMP and ADO production by B cells in the presence of exogenous ATP
| . | 5′AMP . | ADO . | Inosine . | Hypoxanthine . |
|---|---|---|---|---|
| Cells tested . | ng/mL . | |||
| Control | 49; 34 | 0; 0 | 0; 0 | 0; 0 |
| Fresh B cells | 123; 69 | 14; 6 | 0; 0 | 1; 0 |
| Activated B cells | 475; 258 | 8; 4 | 3; 2 | 4; 1 |
| . | 5′AMP . | ADO . | Inosine . | Hypoxanthine . |
|---|---|---|---|---|
| Cells tested . | ng/mL . | |||
| Control | 49; 34 | 0; 0 | 0; 0 | 0; 0 |
| Fresh B cells | 123; 69 | 14; 6 | 0; 0 | 1; 0 |
| Activated B cells | 475; 258 | 8; 4 | 3; 2 | 4; 1 |
For detection of ATP and 5′-AMP hydrolysis by mass spectrometry, 25 000 B cells were incubated in 200 µL PBS in 96-well plates in the presence of 20 µM ATP for 60 minutes. Control wells contained ATP alone. The data are from 2 independent experiments.