Similarities and differences in the experimental setup used to determine the in vivo effect of Stat5 deficiency in BCR-ABL1–induced leukemogenesis
| Experimental setup . | Walz et al . | Hoelbl et al . |
|---|---|---|
| Mouse strain | Balb/c | C57/B6 |
| Time point of STATS deletion | Disease induction | Established disease |
| Viral titer* | Higher | Lower |
| Number of cells injected (intravenously) | 5 × 105 | 1 × 106 |
| Induction of Mx1-Cre transgene | plpC 250 μg (4×) | pIpC 400μg (l×)/IFN-β (1000 U/mL) |
| Age of donor and recipient mice | 6 wk | 6 wk |
| 5-FU treatment dose | 150 mg/kg | 150 mg/kg |
| Retroviral vector | pMSCV-p210-IRES-eGFP | pMSCV-p210-IRES-eGFP |
| Experimental setup . | Walz et al . | Hoelbl et al . |
|---|---|---|
| Mouse strain | Balb/c | C57/B6 |
| Time point of STATS deletion | Disease induction | Established disease |
| Viral titer* | Higher | Lower |
| Number of cells injected (intravenously) | 5 × 105 | 1 × 106 |
| Induction of Mx1-Cre transgene | plpC 250 μg (4×) | pIpC 400μg (l×)/IFN-β (1000 U/mL) |
| Age of donor and recipient mice | 6 wk | 6 wk |
| 5-FU treatment dose | 150 mg/kg | 150 mg/kg |
| Retroviral vector | pMSCV-p210-IRES-eGFP | pMSCV-p210-IRES-eGFP |
IFN-β, interferon β; pIpC, polyinosinic polycytidylic acid; 5-FU, 5-fluorouracil.
V Sexl and R Van Etten, personal communication, 2010.