Early and late apoptosis in leukocytes
| Cells . | Viability . | Control (n = 10) . | Patient V-4 (n = 2) . | P value patient V-4 . | Patient V-9 (n = 4) . | P value patient V-9 . | Patients V-4 + V-9 (n = 6) . | P value V-4 + V-9 . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % gated . | MF . | % gated . | MF . | % gated . | MF . | % gated . | MF . | % gated . | MF . | % gated . | MF . | % gated . | MF . | ||
| Neutrophils | Viable cells | 76 ± 8 | NA | 44 ± 8.6 | NA | .0324 | NA | 21 ± 5 | NA | .0007 | NA | 28 ± 14 | NA | .0002 | NA |
| Early apoptotic cells | 21 ± 7 | 68 ± 18 | 34 ± 6 | 160 ± 51 | .0525 | 0.0668 | 75 ± 4 | 275 ± 16 | .0006 | 0.0002 | 66 ± 23 | 237 ± 69 | .0081 | 0.0009 | |
| Late apoptotic cells | 3 ± 1 | 725 ± 480 | 21 ± 15 | 923 ± 992 | .0960 | 0.0405 | 5 ± 1 | 1376 ± 504 | .0431 | 0.0476 | 6 ± 11 | 1195 ± 660 | .0456 | 0.0276 | |
| Monocytes | Viable cells | 6 ± 5 | NA | 28 ± 7 | NA | .0464 | NA | 17 ± 5 | NA | .0264 | NA | 22 ± 8 | NA | .0153 | NA |
| Early apoptotic cells | 91 ± 6 | 1839 ± 782 | 69 ± 7 | 700 ± 150 | .0960 | 0.0079 | 81 ± 6 | 1071 ± 529 | .2676 | 0.7171 | 75 ± 9 | 886 ± 383 | .0234 | 0.0711 | |
| Late apoptotic cells | 3 ± 1 | 3125 ± 217 | 3 ± 0 | 4635 ± 88 | .5527 | 0.0117 | 2 ± 1 | 3466 ± 851 | .7196 | 0.6179 | 3 ± 1 | 4051 ± 836 | .5503 | 0.0405 | |
| Lymphocytes | Viable cells | 68 ± 15 | NA | 91 ± 4 | NA | .1204 | NA | 91 ± 2 | NA | .2347 | NA | 91 ± 4 | NA | .0223 | NA |
| Early apoptotic cells | 31 ± 15 | 27 ± 20 | 9 ± 4 | 21 ± 8 | .1437 | 0.4893 | 9 ± 2.0 | 11 ± 5 | .2418 | 0.4062 | 9 ± 3 | 16 ± 8 | .0270 | 0.3668 | |
| Late apoptotic cells | 1 ± 1 | 1917 ± 1023 | 1 ± 0 | 2260 ± 1524 | .2065 | 0.8112 | 1 ± 1 | 3747 ± 664 | .9413 | 0.0925 | 0 ± 0 | 3004 ± 1288 | .3097 | 0.0584 | |
| Cells . | Viability . | Control (n = 10) . | Patient V-4 (n = 2) . | P value patient V-4 . | Patient V-9 (n = 4) . | P value patient V-9 . | Patients V-4 + V-9 (n = 6) . | P value V-4 + V-9 . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % gated . | MF . | % gated . | MF . | % gated . | MF . | % gated . | MF . | % gated . | MF . | % gated . | MF . | % gated . | MF . | ||
| Neutrophils | Viable cells | 76 ± 8 | NA | 44 ± 8.6 | NA | .0324 | NA | 21 ± 5 | NA | .0007 | NA | 28 ± 14 | NA | .0002 | NA |
| Early apoptotic cells | 21 ± 7 | 68 ± 18 | 34 ± 6 | 160 ± 51 | .0525 | 0.0668 | 75 ± 4 | 275 ± 16 | .0006 | 0.0002 | 66 ± 23 | 237 ± 69 | .0081 | 0.0009 | |
| Late apoptotic cells | 3 ± 1 | 725 ± 480 | 21 ± 15 | 923 ± 992 | .0960 | 0.0405 | 5 ± 1 | 1376 ± 504 | .0431 | 0.0476 | 6 ± 11 | 1195 ± 660 | .0456 | 0.0276 | |
| Monocytes | Viable cells | 6 ± 5 | NA | 28 ± 7 | NA | .0464 | NA | 17 ± 5 | NA | .0264 | NA | 22 ± 8 | NA | .0153 | NA |
| Early apoptotic cells | 91 ± 6 | 1839 ± 782 | 69 ± 7 | 700 ± 150 | .0960 | 0.0079 | 81 ± 6 | 1071 ± 529 | .2676 | 0.7171 | 75 ± 9 | 886 ± 383 | .0234 | 0.0711 | |
| Late apoptotic cells | 3 ± 1 | 3125 ± 217 | 3 ± 0 | 4635 ± 88 | .5527 | 0.0117 | 2 ± 1 | 3466 ± 851 | .7196 | 0.6179 | 3 ± 1 | 4051 ± 836 | .5503 | 0.0405 | |
| Lymphocytes | Viable cells | 68 ± 15 | NA | 91 ± 4 | NA | .1204 | NA | 91 ± 2 | NA | .2347 | NA | 91 ± 4 | NA | .0223 | NA |
| Early apoptotic cells | 31 ± 15 | 27 ± 20 | 9 ± 4 | 21 ± 8 | .1437 | 0.4893 | 9 ± 2.0 | 11 ± 5 | .2418 | 0.4062 | 9 ± 3 | 16 ± 8 | .0270 | 0.3668 | |
| Late apoptotic cells | 1 ± 1 | 1917 ± 1023 | 1 ± 0 | 2260 ± 1524 | .2065 | 0.8112 | 1 ± 1 | 3747 ± 664 | .9413 | 0.0925 | 0 ± 0 | 3004 ± 1288 | .3097 | 0.0584 | |
Freshly isolated neutrophils, monocytes, and lymphocytes were evaluated for early apoptosis (Annexin + PI−) or late apoptosis (PI+).