| Features of the novel TCR vector . |
|---|
| 1. The lentiviral construct encodes a high-affinity TCR against the antigen and consistently produces highly potent T cells from any suitable donor. |
| 2. The TCR is expressed on both CD8 and on CD4 T cells together with a CD8 coreceptor, so that antigen-driven donor CD4 T cells can provide essential help for the survival of cytotoxic CD8 T cells, thus greatly enhancing their potency. |
| 3. The construct includes a selectable marker so that TCR transgenic T cells can be enriched with enhanced efficacy and safety. |
| 4. The genetically modified population can be selected for memory T cells, thereby excluding naive T cells, which have much greater potential to cause GVHD. |
| 5. The construct includes an inducible caspase 9 suicide switch for ultimate patient safety, in case pathogenic T cells were to cause GVHD. |
| Features of the novel TCR vector . |
|---|
| 1. The lentiviral construct encodes a high-affinity TCR against the antigen and consistently produces highly potent T cells from any suitable donor. |
| 2. The TCR is expressed on both CD8 and on CD4 T cells together with a CD8 coreceptor, so that antigen-driven donor CD4 T cells can provide essential help for the survival of cytotoxic CD8 T cells, thus greatly enhancing their potency. |
| 3. The construct includes a selectable marker so that TCR transgenic T cells can be enriched with enhanced efficacy and safety. |
| 4. The genetically modified population can be selected for memory T cells, thereby excluding naive T cells, which have much greater potential to cause GVHD. |
| 5. The construct includes an inducible caspase 9 suicide switch for ultimate patient safety, in case pathogenic T cells were to cause GVHD. |
Dossa et al address 5 fundamental problems that could hamper the efficacy or safety of T cells against minor histocompatibility antigens.