Binding kinetics of anti–GM-CSF antibodies determined by surface plasmon resonance analysis
| Anti-GM-CSF antibody . | ka, M–1s−1 . | kd, s−1 . | KD, pM . |
|---|---|---|---|
| LMM102 | 7.20 × 105 | 2.2 × 10−5 | 30.5 |
| KB003 | 2.86 × 105 | 7.20 × 10−6 | 25.1 |
| Anti-GM-CSF antibody . | ka, M–1s−1 . | kd, s−1 . | KD, pM . |
|---|---|---|---|
| LMM102 | 7.20 × 105 | 2.2 × 10−5 | 30.5 |
| KB003 | 2.86 × 105 | 7.20 × 10−6 | 25.1 |
ka, association constant (ka); kd, dissociation constant; KD, calculated affinity.
KB003 was captured on anti-F(ab)2–coated surface in a Biacore 3000 instrument. GM-CSF was injected over the surface at multiple concentrations and global fit analysis carried out assuming a 1:1 interaction. Data are means from 2 independent assays at 37°C.