Table 2 Characteristics of primers for... | American Society of Hematology

Table 2

Characteristics of primers for detection of microRNAs and small RNAs

microRNA	Catalog Number*	NCBI Accession	Target Size†	Expression level (Cq)‡
High expression (Cq < 20)
SNORD49A	203904	NR_002744	71 bp	19.0 ± 0.6
SNORD38B	203901	NR_001457	69 bp	19.1 ± 1.0
miR-155	204308	HC_040174	23 bp	19.6 ± 0.8
Intermediate expression (Cq 20-25)
miR-191	204306	HC_040210	23 bp	21.9 ± 0.9
miR-103	204063	HC_885819	23 bp	22.2 ± 0.9
miR-142-5p	204722	HC_040142	21 bp	22.6 ± 1.6
Low expression (Cq > 25)
miR-223	204256	HC_040280	22 bp	25.9 ± 1.5
miR-26b	204172	HC_885768	21 bp	26.5 ± 2.7

microRNA	Catalog Number*	NCBI Accession	Target Size†	Expression level (Cq)‡
High expression (Cq < 20)
SNORD49A	203904	NR_002744	71 bp	19.0 ± 0.6
SNORD38B	203901	NR_001457	69 bp	19.1 ± 1.0
miR-155	204308	HC_040174	23 bp	19.6 ± 0.8
Intermediate expression (Cq 20-25)
miR-191	204306	HC_040210	23 bp	21.9 ± 0.9
miR-103	204063	HC_885819	23 bp	22.2 ± 0.9
miR-142-5p	204722	HC_040142	21 bp	22.6 ± 1.6
Low expression (Cq > 25)
miR-223	204256	HC_040280	22 bp	25.9 ± 1.5
miR-26b	204172	HC_885768	21 bp	26.5 ± 2.7

NCBI, National Center for Biotechnology Information.

Catalog numbers from Exiqon.

PCR amplicon size for all targets is between 60 and 70 bp on agarose gel.

Values represent mean Cq values (with standard deviation) in cells from 3 donors.