Characteristics of primers for detection of mRNA transcripts
| Transcript . | Forward sequence . | Reverse sequence . | Amplicon size . | Expression level (Cq)* . |
|---|---|---|---|---|
| High expression | ||||
| β-actin | 5′-accacaccttctacaatgag-3′ | 5′-tagcacagcctggatagc-3′ | 161 bp | 13.9 ± 1.4 |
| GAPDH | 5′-acccactcctccacctttgac-3′ | 5′-tccaccaccctgttgctgtag-3′ | 110 bp | 15.1 ± 1.4 |
| Intermediate expression | ||||
| TGF-β1 | 5′-cccagcatctgcaaagctc-3′ | 5′-gtcaatgtacagctgccgca-3′ | 101 bp | 18.6 ± 0.8 |
| Low expression | ||||
| CD25 | 5′-gactgctcacgttcatcatggt-3′ | 5′-aatgtggcgtgtgggatctc-3′ | 82 bp | 20.8 ± 2.8 |
| HPRT-1 | 5′-agatggtcaaggtcgcaagc-3′ | 5′-tcaagggcatatcctacaacaaac-3′ | 165 bp | 21.7 ± 2.6 |
| Foxp3 | 5′-acagcacattcccagagttc-3′ | 5′-caggtggcaggatggtttc-3′ | 190 bp | 21.9 ± 1.4 |
| Transcript . | Forward sequence . | Reverse sequence . | Amplicon size . | Expression level (Cq)* . |
|---|---|---|---|---|
| High expression | ||||
| β-actin | 5′-accacaccttctacaatgag-3′ | 5′-tagcacagcctggatagc-3′ | 161 bp | 13.9 ± 1.4 |
| GAPDH | 5′-acccactcctccacctttgac-3′ | 5′-tccaccaccctgttgctgtag-3′ | 110 bp | 15.1 ± 1.4 |
| Intermediate expression | ||||
| TGF-β1 | 5′-cccagcatctgcaaagctc-3′ | 5′-gtcaatgtacagctgccgca-3′ | 101 bp | 18.6 ± 0.8 |
| Low expression | ||||
| CD25 | 5′-gactgctcacgttcatcatggt-3′ | 5′-aatgtggcgtgtgggatctc-3′ | 82 bp | 20.8 ± 2.8 |
| HPRT-1 | 5′-agatggtcaaggtcgcaagc-3′ | 5′-tcaagggcatatcctacaacaaac-3′ | 165 bp | 21.7 ± 2.6 |
| Foxp3 | 5′-acagcacattcccagagttc-3′ | 5′-caggtggcaggatggtttc-3′ | 190 bp | 21.9 ± 1.4 |
Values represent mean Cq values (with standard deviation) in cells from 4 donors.