Table 1

Selectin ligand function and leukocyte rolling in vitro and in vivo in St3gal4-, St3gal6-, and double-deficient mice compared with wild-type mice

St3gal4Δ/ΔSt3gal6Δ/ΔSt3gal4Δ/ΔSt3gal6Δ/Δ
In vitro 
    P-selectin binding to neutrophils ↓ ↓ ↓↓ 
    E-selectin binding to neutrophils ↓↓ ↓ ↓↓↓ 
    L-selectin binding to secondary lymphatic tissues ↓ = − ↓ ↓↓ 
    P-selectin–dependent rolling (flow chamber) ↓ 16  ↓ ↓↓↓ 
    E-selectin–dependent rolling (flow chamber) ↓ 16  = ↓↓↓ 
In vivo 
    P-selectin–dependent rolling = 16  ↓↓ ↓↓ 
    P-selectin–dependent rolling velocities (TNF-α–stimulated cremaster muscle) = 16  = = 
    E-selectin–dependent rolling ↓ 16  = ↓↓↓ 
    E-selectin–dependent rolling velocities (TNF-α–stimulated cremaster muscle) ↑↑ = ↑↑ 
    Lymphocyte homing (adoptive transfer of wild-type B and T cells) ↓ = ↓↓ 
St3gal4Δ/ΔSt3gal6Δ/ΔSt3gal4Δ/ΔSt3gal6Δ/Δ
In vitro 
    P-selectin binding to neutrophils ↓ ↓ ↓↓ 
    E-selectin binding to neutrophils ↓↓ ↓ ↓↓↓ 
    L-selectin binding to secondary lymphatic tissues ↓ = − ↓ ↓↓ 
    P-selectin–dependent rolling (flow chamber) ↓ 16  ↓ ↓↓↓ 
    E-selectin–dependent rolling (flow chamber) ↓ 16  = ↓↓↓ 
In vivo 
    P-selectin–dependent rolling = 16  ↓↓ ↓↓ 
    P-selectin–dependent rolling velocities (TNF-α–stimulated cremaster muscle) = 16  = = 
    E-selectin–dependent rolling ↓ 16  = ↓↓↓ 
    E-selectin–dependent rolling velocities (TNF-α–stimulated cremaster muscle) ↑↑ = ↑↑ 
    Lymphocyte homing (adoptive transfer of wild-type B and T cells) ↓ = ↓↓ 

The number of arrows indicates the magnitude of the change.

↑ indicates increases; ↓, decreases; =, no significant changes.

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