Information on sequences and source of synthetic oligos of a phosphodiester DNA backbone and plasmids used in studies of LL37-DNA binding affinity
| DNA type . | DNA sequence . | Source . |
|---|---|---|
| Poly A | 5′ AAA AAA AAA AAA AAA 3′ | Trilink |
| Poly T | 5′ TTT TTT TTT TTT TTT 3′ | Trilink |
| CpG DNA (sense) | 5′ XTC GTC GTT TTG TCG TTT TGT CGT TG 3′; | Trilink |
| CpG DNA (antisense) | 5′ XAA CGA CAA AAC GAC AAA ACG ACG AG 3′ | Trilink |
| GpC DNA (sense) | 5′ XTG CTG CTT TTG TGC TTT TGT GCT TG 3′; | Trilink |
| GpC DNA (antisense) | 5′ XAA GCA CAA AAG CAC AAA AGC AGC AG 3′ | Trilink |
| CpG DNA biotin (5′ end; sense) | 5′ TC GTC GTT TTG TCG TTT TGT CGTT 3′ | Sigma-Aldrich |
| CpG DNA (antisense) | 5′ AA CGA CAA AAC GAC AAA ACG ACGA 3′ | Sigma-Aldrich |
| Poly dA:dT | poly (dA:dT) ̇ poly (dT:dA), synthetic phosphodiester ds DNA polymer | Sigma-Aldrich |
| Poly dC:dG | poly (dC:dG) ̇ poly (dG:dC), synthetic phosphodiester ds DNA polymer | Sigma-Aldrich |
| CpG containing plasmid | pBR322 plasmid, 4361 base pairs, contains 297 unmethylated CG base pairs | Invitrogen |
| CpG free plasmid | pCpG-mcs G2, 3066 base pairs, does not contain any CG base pairs | Invivogen |
| DNA type . | DNA sequence . | Source . |
|---|---|---|
| Poly A | 5′ AAA AAA AAA AAA AAA 3′ | Trilink |
| Poly T | 5′ TTT TTT TTT TTT TTT 3′ | Trilink |
| CpG DNA (sense) | 5′ XTC GTC GTT TTG TCG TTT TGT CGT TG 3′; | Trilink |
| CpG DNA (antisense) | 5′ XAA CGA CAA AAC GAC AAA ACG ACG AG 3′ | Trilink |
| GpC DNA (sense) | 5′ XTG CTG CTT TTG TGC TTT TGT GCT TG 3′; | Trilink |
| GpC DNA (antisense) | 5′ XAA GCA CAA AAG CAC AAA AGC AGC AG 3′ | Trilink |
| CpG DNA biotin (5′ end; sense) | 5′ TC GTC GTT TTG TCG TTT TGT CGTT 3′ | Sigma-Aldrich |
| CpG DNA (antisense) | 5′ AA CGA CAA AAC GAC AAA ACG ACGA 3′ | Sigma-Aldrich |
| Poly dA:dT | poly (dA:dT) ̇ poly (dT:dA), synthetic phosphodiester ds DNA polymer | Sigma-Aldrich |
| Poly dC:dG | poly (dC:dG) ̇ poly (dG:dC), synthetic phosphodiester ds DNA polymer | Sigma-Aldrich |
| CpG containing plasmid | pBR322 plasmid, 4361 base pairs, contains 297 unmethylated CG base pairs | Invitrogen |
| CpG free plasmid | pCpG-mcs G2, 3066 base pairs, does not contain any CG base pairs | Invivogen |