Table 2 Affinity of the PCET/KIX... | American Society of Hematology

Table 2

Affinity of the PCET/KIX interaction

Ligand	K_d, μM
PCET peptides titrated with KIX>
E2A-PCET	12 ± 2*
HEB-PCET	9.4 ± 0.1*
E2A-PCET Leu20Ala	ND†
E2A-PCET Phe22Ala	71 ± 2†
HEB-PCET Glu16Ala	25 ± 1*
HEB-PCET Asp22Ala	25 ± 1*
KIX constructs titrated into HEB-PCET
KIX	9.4 ± 0.1*
KIX Lys667Ala	28 ± 2*
KIX Lys667Glu	95 ± 1*
KIX Phe612Ala/Asp622Ala/Arg624Ala/Lys667Ala	204 ± 7*

Ligand	K_d, μM
PCET peptides titrated with KIX>
E2A-PCET	12 ± 2*
HEB-PCET	9.4 ± 0.1*
E2A-PCET Leu20Ala	ND†
E2A-PCET Phe22Ala	71 ± 2†
HEB-PCET Glu16Ala	25 ± 1*
HEB-PCET Asp22Ala	25 ± 1*
KIX constructs titrated into HEB-PCET
KIX	9.4 ± 0.1*
KIX Lys667Ala	28 ± 2*
KIX Lys667Glu	95 ± 1*
KIX Phe612Ala/Asp622Ala/Arg624Ala/Lys667Ala	204 ± 7*

ND indicates no detectable binding.

K_d values determined by fluorescence anisotropy titration.

K_d values determined by isothermal titration calorimetry.