Mouse models of hematologic malignancies characterized by KRAS, NRAS, or NF1 mutations
| Allele . | Model type . | Genotype . | Phenotype(s) . | References . |
|---|---|---|---|---|
| KrasG12D | Heterozygous conditional “knock in” | Mx1-Cre; KrasG12D/+ | Aggressive MPN | 35, 56 |
| KrasG12D | Adoptive transfer of conditional “knock in” marrow into irradiated recipients | Mx1-Cre; KrasG12D/+ | T-ALL | 65, 108 |
| KrasG12D | Conditional “knock in” + retroviral insertional mutagenesis | Mx1-Cre; KrasG12D/+ | T-ALL (∼ 80%); AML (∼ 20%) | 66 |
| NrasG12D | Retroviral transduction/transplantation | WT cells expressing exogenous NrasG12D | CMML, AML | 69, 70, 71 |
| NrasG12D | Heterozygous conditional “knock in” | Mx1-Cre; NrasG12D/+ | Indolent MPN, MDS, LPD, HS | 57, 58, 75 |
| NrasG12D | Homozygous conditional “knock in” | Mx1-Cre; NrasG12D/G12D | Aggressive MPN | 59, 60 |
| NrasG12D | Heterozygous conditional “knock in” + retroviral insertional mutagenesis | Mx1-Cre; NrasG12D/+ | AML | 58 |
| Nf1− | Heterozygous germline “knock out” | Nf1+/− | Late-onset MPN in ∼ 10% of mice | 109 |
| Nf1− | Adoptive transfer of homozygous “knock out” fetal liver cells | Nf1−/− | Subactute MPN | 110 |
| Nf1− | Heterozygous “knock out” + irradiation | Nf1+/− | MDS, MPN | 72, 73 |
| Nf1flox | Homozygous conditional “knock out” | Mx1-Cre; Nf1flox/flox | MPN | 74 |
| Nf1flox | Homozygous conditional “knock out” + retroviral insertional mutagenesis | Mx1-Cre; Nf1flox/flox | AML (∼ 70%); T-ALL (∼ 30%) | 19 |
| Allele . | Model type . | Genotype . | Phenotype(s) . | References . |
|---|---|---|---|---|
| KrasG12D | Heterozygous conditional “knock in” | Mx1-Cre; KrasG12D/+ | Aggressive MPN | 35, 56 |
| KrasG12D | Adoptive transfer of conditional “knock in” marrow into irradiated recipients | Mx1-Cre; KrasG12D/+ | T-ALL | 65, 108 |
| KrasG12D | Conditional “knock in” + retroviral insertional mutagenesis | Mx1-Cre; KrasG12D/+ | T-ALL (∼ 80%); AML (∼ 20%) | 66 |
| NrasG12D | Retroviral transduction/transplantation | WT cells expressing exogenous NrasG12D | CMML, AML | 69, 70, 71 |
| NrasG12D | Heterozygous conditional “knock in” | Mx1-Cre; NrasG12D/+ | Indolent MPN, MDS, LPD, HS | 57, 58, 75 |
| NrasG12D | Homozygous conditional “knock in” | Mx1-Cre; NrasG12D/G12D | Aggressive MPN | 59, 60 |
| NrasG12D | Heterozygous conditional “knock in” + retroviral insertional mutagenesis | Mx1-Cre; NrasG12D/+ | AML | 58 |
| Nf1− | Heterozygous germline “knock out” | Nf1+/− | Late-onset MPN in ∼ 10% of mice | 109 |
| Nf1− | Adoptive transfer of homozygous “knock out” fetal liver cells | Nf1−/− | Subactute MPN | 110 |
| Nf1− | Heterozygous “knock out” + irradiation | Nf1+/− | MDS, MPN | 72, 73 |
| Nf1flox | Homozygous conditional “knock out” | Mx1-Cre; Nf1flox/flox | MPN | 74 |
| Nf1flox | Homozygous conditional “knock out” + retroviral insertional mutagenesis | Mx1-Cre; Nf1flox/flox | AML (∼ 70%); T-ALL (∼ 30%) | 19 |