Microarray analysis of Wnt3a-transduced OP9 cells
| . | Symbol . | Sequence description . | Accession no. . | Fold change . | P . |
|---|---|---|---|---|---|
| MSC | Nes | Nestin | NM_016701 | −1.17 | .362 |
| Eng | Endoglin | NM_007932 | −1.11 | .554 | |
| Pdgfra | Platelet derived growth factor receptor, alpha polypeptide | NM_011058 | −5.69 | <.001 | |
| Thy1 | Thymus cell antigen 1, CD90 | NM_009382 | −2.21 | <.001 | |
| Osteoblastic | Alp | Alkaline phosphatase, liver/bone/kidney | NM_007431 | > 100 | <.001 |
| Runx2 | Runt related transcription factor 2 | NM_009820 | 4.64 | <.001 | |
| Bmp4 | Bone morphogenetic protein 4 | NM_007554 | 2.02 | <.001 | |
| Ogn | Osteoglycin | NM_008760 | 3.22 | <.001 | |
| Sp7 | Sp7 transcription factor 7, osterix | NM_130458 | 22.45 | <.001 | |
| Bglap1 | Bone gamma carboxyglutamate protein 1, osteocalcin | NM_007541 | 1.98 | .020 | |
| Gpnmb | Glycoprotein (transmembrane) nmb, osteoactivin | NM_053110 | 3.85 | <.001 | |
| Pth1r | Parathyroid hormone 1 receptor | NM_011199 | 33.48 | <.001 | |
| Col1a1 | Collagen, type I, alpha 1 | NM_007742 | 4.69 | <.001 | |
| Adipogenic | Cfd | Complement factor D, adipsin | NM_013459 | −1.71 | .230 |
| Pparg | Peroxisome proliferator activated receptor gamma | NM_011146 | −6.53 | <.001 | |
| Fabp4 | Fatty acid binding protein 4, Ap2 | NM_024406 | −13.34 | <.001 | |
| Plin | Perilipin | NM_175640 | −3.07 | <.001 | |
| Chondrogenic | Acan | Aggrecan | NM_007424 | −1.13 | .172 |
| Sox9 | SRY-box containing gene 9 | NM_011448 | −2.96 | <.001 | |
| Col2a1 | Collagen, type II, alpha 1 | NM_031163 | −3.93 | <.001 | |
| Col11a2 | Collagen, type XI, alpha 2 | NM_009926 | −1.26 | .390 | |
| HSC-supportive | Kitl | Kit ligand | NM_013598 | −27.53 | <.001 |
| Vcam1 | Vascular cell adhesion molecule 1 | NM_011693 | −10.21 | <.001 | |
| Cxcl12 | Chemokine (C-X-C motif) ligand 12 | NM_021704 | −4.22 | <.001 | |
| Angpt1 | Angiopoietin 1 | NM_009640 | <−100 | <.001 | |
| Wnt target | Axin2 | Axin2 | NM_015732 | 11.61 | <.001 |
| Myc | Myelocytomatosis oncogene | NM_010849 | 2.48 | <.001 | |
| Bmp4 | Bone morphogenetic protein 4 | NM_007554 | 2.02 | <.001 | |
| Ptgs2 | Prostaglandin-endoperoxide synthase 2 | NM_011198 | 2.14 | .015 | |
| Fn1 | Fibronectin 1 | NM_010233 | 1.07 | .593 | |
| Membrane/ECM protein | Dcn | Decorin | NM_007833 | 57.67 | <.001 |
| Bgn | Biglycan | NM_007542 | −1.18 | .057 | |
| Fmod | Fibromodulin | NM_021355 | > 100 | <.001 | |
| Tnc | Tenascin C | NM_011607 | > 100 | <.001 | |
| Tnn | Tenascin N | NM_177839 | > 100 | <.001 | |
| Tnxb | Tenascin XB | NM_031176 | −10.69 | <.001 | |
| Ncam1 | Neural cell adhesion molecule 1 | NM_010875 | 14.88 | <.001 | |
| Clec11a | C-type lectin domain family 11, member a, stem cell growth factor (SCGF) | NM_009131 | 85.60 | <.001 |
| . | Symbol . | Sequence description . | Accession no. . | Fold change . | P . |
|---|---|---|---|---|---|
| MSC | Nes | Nestin | NM_016701 | −1.17 | .362 |
| Eng | Endoglin | NM_007932 | −1.11 | .554 | |
| Pdgfra | Platelet derived growth factor receptor, alpha polypeptide | NM_011058 | −5.69 | <.001 | |
| Thy1 | Thymus cell antigen 1, CD90 | NM_009382 | −2.21 | <.001 | |
| Osteoblastic | Alp | Alkaline phosphatase, liver/bone/kidney | NM_007431 | > 100 | <.001 |
| Runx2 | Runt related transcription factor 2 | NM_009820 | 4.64 | <.001 | |
| Bmp4 | Bone morphogenetic protein 4 | NM_007554 | 2.02 | <.001 | |
| Ogn | Osteoglycin | NM_008760 | 3.22 | <.001 | |
| Sp7 | Sp7 transcription factor 7, osterix | NM_130458 | 22.45 | <.001 | |
| Bglap1 | Bone gamma carboxyglutamate protein 1, osteocalcin | NM_007541 | 1.98 | .020 | |
| Gpnmb | Glycoprotein (transmembrane) nmb, osteoactivin | NM_053110 | 3.85 | <.001 | |
| Pth1r | Parathyroid hormone 1 receptor | NM_011199 | 33.48 | <.001 | |
| Col1a1 | Collagen, type I, alpha 1 | NM_007742 | 4.69 | <.001 | |
| Adipogenic | Cfd | Complement factor D, adipsin | NM_013459 | −1.71 | .230 |
| Pparg | Peroxisome proliferator activated receptor gamma | NM_011146 | −6.53 | <.001 | |
| Fabp4 | Fatty acid binding protein 4, Ap2 | NM_024406 | −13.34 | <.001 | |
| Plin | Perilipin | NM_175640 | −3.07 | <.001 | |
| Chondrogenic | Acan | Aggrecan | NM_007424 | −1.13 | .172 |
| Sox9 | SRY-box containing gene 9 | NM_011448 | −2.96 | <.001 | |
| Col2a1 | Collagen, type II, alpha 1 | NM_031163 | −3.93 | <.001 | |
| Col11a2 | Collagen, type XI, alpha 2 | NM_009926 | −1.26 | .390 | |
| HSC-supportive | Kitl | Kit ligand | NM_013598 | −27.53 | <.001 |
| Vcam1 | Vascular cell adhesion molecule 1 | NM_011693 | −10.21 | <.001 | |
| Cxcl12 | Chemokine (C-X-C motif) ligand 12 | NM_021704 | −4.22 | <.001 | |
| Angpt1 | Angiopoietin 1 | NM_009640 | <−100 | <.001 | |
| Wnt target | Axin2 | Axin2 | NM_015732 | 11.61 | <.001 |
| Myc | Myelocytomatosis oncogene | NM_010849 | 2.48 | <.001 | |
| Bmp4 | Bone morphogenetic protein 4 | NM_007554 | 2.02 | <.001 | |
| Ptgs2 | Prostaglandin-endoperoxide synthase 2 | NM_011198 | 2.14 | .015 | |
| Fn1 | Fibronectin 1 | NM_010233 | 1.07 | .593 | |
| Membrane/ECM protein | Dcn | Decorin | NM_007833 | 57.67 | <.001 |
| Bgn | Biglycan | NM_007542 | −1.18 | .057 | |
| Fmod | Fibromodulin | NM_021355 | > 100 | <.001 | |
| Tnc | Tenascin C | NM_011607 | > 100 | <.001 | |
| Tnn | Tenascin N | NM_177839 | > 100 | <.001 | |
| Tnxb | Tenascin XB | NM_031176 | −10.69 | <.001 | |
| Ncam1 | Neural cell adhesion molecule 1 | NM_010875 | 14.88 | <.001 | |
| Clec11a | C-type lectin domain family 11, member a, stem cell growth factor (SCGF) | NM_009131 | 85.60 | <.001 |
Microarray analyses were conducted comparing Wnt3a with vector-transduced OP9 stromal cells, and the average probe intensities from 2 independent samples were used to calculate the -fold change.