Effects of PGE2 inhibition on bone marrow cellularity
| Group* . | WBC/femur† . | Neutrophils‡ . | Monocytes‡ . | B cells‡ . | T + NK cells‡ . |
|---|---|---|---|---|---|
| Vehicle | 17.0 ± 0.9 | 6. ± 0.3 (37.6 ± 1.0) | 1.8 ± 0.2 (10.0 ± 0.7) | 4.0 ± 0.2 (27.7 ± 0.3) | 3.7 ± 0.5 (27.6 ± 1.0) |
| Indomethacin | 17.3 ± 1.3 | 6.3 ± 0.4 (38.0 ± 1.1) | 2.5 ± 0.2§ (14.6 ± 0.3) | 3.4 ± 0.4 (21.6 ± 2.4) | 4.0 ± 0.5 (25.6 ± 1.2) |
| Group* . | WBC/femur† . | Neutrophils‡ . | Monocytes‡ . | B cells‡ . | T + NK cells‡ . |
|---|---|---|---|---|---|
| Vehicle | 17.0 ± 0.9 | 6. ± 0.3 (37.6 ± 1.0) | 1.8 ± 0.2 (10.0 ± 0.7) | 4.0 ± 0.2 (27.7 ± 0.3) | 3.7 ± 0.5 (27.6 ± 1.0) |
| Indomethacin | 17.3 ± 1.3 | 6.3 ± 0.4 (38.0 ± 1.1) | 2.5 ± 0.2§ (14.6 ± 0.3) | 3.4 ± 0.4 (21.6 ± 2.4) | 4.0 ± 0.5 (25.6 ± 1.2) |
WBC indicates white blood cell; and NK, natural killer.
Groups of 5 mice were treated with vehicle (0.01% ethanol in PBS) or indomethacin (2.5 mg/kg) twice daily for 6 days.
BM cells were harvested from vehicle control and indomethacin-treated mice, and WBCs were determined on individual mice with the use of a Hemavet 950 analyzer. WBCs are expressed as mean ± SEM total nucleated cells per femur × 106, based on counts from individual mice. Data are representative of 5 experiments.
Harvested BM cells from treated mice were stained with antibodies for GR-1, Mac1, B220, CD3, and NK1.1 and analyzed by flow cytometry. Data are expressed as mean ± SEM total cells per femur × 106 (mean percentage of WBCs ± SEM), based on analysis of individual mice. Data are representative of 3 experiments.
P < .05