Lin−CD122+NK1.1−DX5− fetal liver NKPs lack myeloid potential
| Experiment . | Cell population . | No. of cells plated per well . | No. of wells with small size clones (3-50 cells) . | No. of wells with medium size clones (50 cells; 10%) . | No. of wells with big clones > 10% . | Total no. of wells with clones . |
|---|---|---|---|---|---|---|
| 1 | NKP | 5 | 0 | 0 | 0 | 0/120 |
| 1 | Lin−CD122− | 10 | 11 | 6 | 35 | 52/60 |
| 2 | NKP | 5-10 | 0 | 0 | 0 | 0/240 |
| 2 | Lin−CD122− | 10 | 7 | 5 | 39 | 51/60 |
| Experiment . | Cell population . | No. of cells plated per well . | No. of wells with small size clones (3-50 cells) . | No. of wells with medium size clones (50 cells; 10%) . | No. of wells with big clones > 10% . | Total no. of wells with clones . |
|---|---|---|---|---|---|---|
| 1 | NKP | 5 | 0 | 0 | 0 | 0/120 |
| 1 | Lin−CD122− | 10 | 11 | 6 | 35 | 52/60 |
| 2 | NKP | 5-10 | 0 | 0 | 0 | 0/240 |
| 2 | Lin−CD122− | 10 | 7 | 5 | 39 | 51/60 |
Indicated cell populations were sorted from fetal liver at E18.5 and plated at the specified cell numbers into wells containing X-vivo medium containing 10% FCS and cytokines: KL+GM-CSF+G-CSF+IL-3+FL+TPO. The plates were scored at day 7 (not shown) and day 12 (data in the table) of the culture with similar results.